Departments of Comparative Medicine, Stanford University, 3172 Porter Drive, Palo Alto, CA 94304-5475, USA.
Departments of Comparative Medicine, Stanford University, 3172 Porter Drive, Palo Alto, CA 94304-5475, USA.
Epilepsy Res. 2024 Aug;204:107384. doi: 10.1016/j.eplepsyres.2024.107384. Epub 2024 Jun 13.
At least 3 months after systemic treatment with pilocarpine to induce status epilepticus, Long-Evans and Sprague-Dawley rats were video-EEG monitored for seizures continuously for 1 month. Rats were then perfused, hippocampi were processed for Nissl staining, and hilar neurons were quantified. Seizure frequency in Long-Evans rats was 1/10th of that in Sprague-Dawley rats, and more variable. Hilar neuron loss was also less severe in Long-Evans rats. However, there was no correlation between hilar neuron loss and seizure frequency in either strain. The low and variable seizure frequency suggests limited usefulness of pilocarpine-treated Long-Evans rats for some epilepsy experiments.
至少在全身性给予毛果芸香碱以诱导癫痫持续状态 3 个月后，对长爪沙鼠和 Sprague-Dawley 大鼠进行视频-EEG 监测，持续 1 个月以监测癫痫发作。然后对大鼠进行灌注，处理海马进行尼氏染色，并对颗粒细胞进行定量。长爪沙鼠的癫痫发作频率是 Sprague-Dawley 大鼠的 1/10，且更具变异性。长爪沙鼠的颗粒细胞丢失也较轻。然而，在两种品系中，颗粒细胞丢失与癫痫发作频率之间均无相关性。低且多变的癫痫发作频率表明，毛果芸香碱处理的长爪沙鼠对某些癫痫实验的用处有限。
