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匹鲁卡品全身治疗后癫痫发作频率和海马神经元丢失的大鼠品系差异。

Rat strain differences in seizure frequency and hilar neuron loss after systemic treatment with pilocarpine.

机构信息

Departments of Comparative Medicine, Stanford University, 3172 Porter Drive, Palo Alto, CA 94304-5475, USA.

Departments of Comparative Medicine, Stanford University, 3172 Porter Drive, Palo Alto, CA 94304-5475, USA.

出版信息

Epilepsy Res. 2024 Aug;204:107384. doi: 10.1016/j.eplepsyres.2024.107384. Epub 2024 Jun 13.

Abstract

At least 3 months after systemic treatment with pilocarpine to induce status epilepticus, Long-Evans and Sprague-Dawley rats were video-EEG monitored for seizures continuously for 1 month. Rats were then perfused, hippocampi were processed for Nissl staining, and hilar neurons were quantified. Seizure frequency in Long-Evans rats was 1/10th of that in Sprague-Dawley rats, and more variable. Hilar neuron loss was also less severe in Long-Evans rats. However, there was no correlation between hilar neuron loss and seizure frequency in either strain. The low and variable seizure frequency suggests limited usefulness of pilocarpine-treated Long-Evans rats for some epilepsy experiments.

摘要

至少在全身性给予毛果芸香碱以诱导癫痫持续状态 3 个月后,对长爪沙鼠和 Sprague-Dawley 大鼠进行视频-EEG 监测,持续 1 个月以监测癫痫发作。然后对大鼠进行灌注,处理海马进行尼氏染色,并对颗粒细胞进行定量。长爪沙鼠的癫痫发作频率是 Sprague-Dawley 大鼠的 1/10,且更具变异性。长爪沙鼠的颗粒细胞丢失也较轻。然而,在两种品系中,颗粒细胞丢失与癫痫发作频率之间均无相关性。低且多变的癫痫发作频率表明,毛果芸香碱处理的长爪沙鼠对某些癫痫实验的用处有限。

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