Stackpole C W, Alterman A L, Fornabaio D M
Invasion Metastasis. 1985;5(3):125-43.
Three distinct dissemination-related phenotypes have been distinguished among cell subpopulations of the mouse B16 melanoma: tumorigenicity, spontaneous metastasis from subcutaneous tumors, and organ colonization following intravenous injection of cells. From a progenitor clone (G3) of tumorigenic but nonmetastatic and noncolonizing (null) cells that underwent phenotypic diversification in vitro and in vivo, 4 subclones were obtained: G3.5 (culture-generated metastatic), G3.12 (tumor-generated metastatic), G3.15 (culture-generated null), and G3.26 (tumor-generated colonizing). The growth potentials of the parent clone and derived subclones were investigated comparatively in in vivo assays (tumorigenicity, tumor growth rate, and lung colonization potential), monolayer culture assays (generation time, saturation density, clonogenicity, and rate of detachment by trypsin), and in soft agar. In overall growth potential, G3.26 greater than G3.12 greater than G3, G3.5 greater than G3.15. These results indicate that metastatic populations of the B16 melanoma are not the most rapidly and effectively growing cells obtainable from that tumor.
在小鼠B16黑色素瘤的细胞亚群中已区分出三种与播散相关的不同表型：致瘤性、皮下肿瘤的自发转移以及静脉注射细胞后的器官定植。从一个在体外和体内经历了表型多样化的致瘤但不转移且不定植（无转移能力）的细胞祖代克隆（G3）中，获得了4个子克隆：G3.5（培养产生的转移性克隆）、G3.12（肿瘤产生的转移性克隆）、G3.15（培养产生的无转移能力克隆）和G3.26（肿瘤产生的定植性克隆）。在体内试验（致瘤性、肿瘤生长速率和肺定植潜力）、单层培养试验（代时、饱和密度、克隆形成能力和胰蛋白酶消化脱落速率）以及软琼脂试验中，对亲本克隆和衍生的子克隆的生长潜力进行了比较研究。在总体生长潜力方面，G3.26＞G3.12＞G3，G3.5＞G3.15。这些结果表明，B16黑色素瘤的转移群体并非从该肿瘤中可获得的生长最快且最有效的细胞。
