在沙门氏菌/哺乳动物微粒体试验中对没食子酸和单宁酸进行的致突变性测试。

Mutagenicity tests with gallic and tannic acid in the Salmonella/mammalian microsome assay.

作者信息

Rashid K A, Baldwin I T, Babish J G, Schultz J C, Mumma R O

出版信息

J Environ Sci Health B. 1985 Apr;20(2):153-65. doi: 10.1080/03601238509372473.

Abstract

Gallic acid, tannic acid mixture and a purified fraction of tannic acid were evaluated for possible mutagenic activity in three strains of Salmonella typhimurium, TA98, TA100, and TA1535. These chemicals were not mutagenic either before or after activation with rat and woodchuck microsomal and cytosolic enzymes. However, tannic acid mixture and tannic acid fraction both gave a significantly (p = 0.05) dose-related reduction in the number of the revertant colonies, compared to the normal spontaneous revertants with no apparent toxic effects in the background lawn. With an agar diffusion assay, the chemicals exhibited toxic effects at 5000 micrograms/disc.

摘要

对没食子酸、单宁酸混合物及单宁酸的一个纯化级分，在鼠伤寒沙门氏菌TA98、TA100和TA1535三株菌株中评估其可能的诱变活性。在用大鼠和土拨鼠微粒体及胞质酶活化之前或之后，这些化学物质均无致突变性。然而，与背景菌苔中无明显毒性作用的正常自发回复突变体相比，单宁酸混合物和单宁酸级分二者均使回复突变菌落数出现显著（p = 0.05）的剂量相关减少。通过琼脂扩散试验，这些化学物质在5000微克/圆片时表现出毒性作用。