Laboratory of Molecular Sensory Science, School of Food and Health, Beijing Technology and Business University (BTBU), Beijing 100048, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Molecules. 2024 May 28;29(11):2537. doi: 10.3390/molecules29112537.
Beer is a popular alcoholic beverage worldwide. However, limited research has been conducted on identifying key odor-active components in lager-type draft beers for the Chinese market. Therefore, this study aims to elucidate the odor characteristics of the four most popular draft beer brands through a sensory evaluation and an electronic nose. Subsequently, the four draft beers were analyzed through solid-phase microextraction and liquid-liquid extraction using a two-dimensional comprehensive gas chromatography-olfactometry-mass spectrometry analysis (GC×GC-O-MS). Fifty-five volatile odor compounds were detected through GC×GC-O-MS. Through an Aroma Extract Dilution Analysis, 22 key odor-active compounds with flavor dilution factors ≥ 16 were identified, with 11 compounds having odor activity values > one. An electronic nose analysis revealed significant disparities in the odor characteristics of the four samples, enabling their distinct identification. These findings help us to better understand the flavor characteristics of draft beer and the stylistic differences between different brands of products and provide a theoretical basis for objectively evaluating the quality differences between different brands of draft beer.
啤酒是一种在全世界范围内广受欢迎的酒精饮料。然而,针对中国市场的拉格型生啤酒中关键气味活性成分的研究还很有限。因此,本研究旨在通过感官评价和电子鼻阐明四种最受欢迎的生啤酒品牌的气味特征。随后,通过固相微萃取和液液萃取,使用二维全气相色谱-嗅闻-质谱联用分析(GC×GC-O-MS)对四种生啤酒进行分析。通过 GC×GC-O-MS 检测到 55 种挥发性气味化合物。通过香气萃取稀释分析,确定了 22 种风味稀释因子≥16 的关键气味活性化合物,其中 11 种化合物的气味活度值大于 1。电子鼻分析表明,这四种样品的气味特征存在显著差异,能够对其进行区分。这些发现有助于我们更好地了解生啤酒的风味特征以及不同品牌产品之间的风格差异,并为客观评价不同品牌生啤酒之间的质量差异提供理论依据。