Department of Life Science and Agroforestry, Qiqihar University, No. 42 Wenhua Street, Jianhua District, Qiqihar, 161000, PR China.
Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province, 161000, PR China.
J Muscle Res Cell Motil. 2024 Dec;45(4):253-262. doi: 10.1007/s10974-024-09677-5. Epub 2024 Jun 19.
The proliferation and differentiation of skeletal muscle satellite cells is a complex physiological process involving various transcription factors and small RNA molecules. This study aimed to understand the regulatory mechanisms underlying these processes, focusing on interferon-related development factor 2 (IFRD2) as a target gene of miRNA-2400 in bovine skeletal MuSCs (MuSCs). IFRD2 was identified as a target gene of miRNA-2400 involved in regulating the proliferation and differentiation of bovine skeletal MuSCs. Our results indicate that miR-2400 can target binding the 3'UTR of IFRD2 and inhibit its translation. mRNA and protein expression levels of IFRD2 increased significantly with increasing days of differentiation. Moreover, overexpression of the IFRD2 gene inhibited proliferation and promoted differentiation of bovine MuSCs. Conversely, the knockdown of the gene had the opposite effect. Overexpression of IFRD2 resulted in the inhibition of ERK1/2 phosphorylation levels in bovine MuSCs, which in turn promoted differentiation. In summary, IFRD2, as a target gene of miR-2400, crucially affects bovine skeletal muscle proliferation and differentiation by precisely regulating ERK1/2 phosphorylation.
骨骼肌卫星细胞的增殖和分化是一个复杂的生理过程，涉及多种转录因子和小 RNA 分子。本研究旨在探讨这些过程的调控机制，重点关注干扰素相关发育因子 2 (IFRD2) 作为牛骨骼肌 MuSCs (MuSCs) 中 miRNA-2400 的靶基因。IFRD2 被鉴定为 miRNA-2400 的靶基因，参与调节牛骨骼肌 MuSCs 的增殖和分化。我们的结果表明，miR-2400 可以靶向结合 IFRD2 的 3'UTR 并抑制其翻译。IFRD2 的 mRNA 和蛋白表达水平随着分化天数的增加而显著增加。此外，IFRD2 基因的过表达抑制了牛 MuSCs 的增殖并促进了分化。相反，基因的敲低则产生相反的效果。IFRD2 的过表达导致牛 MuSCs 中 ERK1/2 磷酸化水平的抑制，从而促进分化。总之，IFRD2 作为 miR-2400 的靶基因，通过精确调节 ERK1/2 磷酸化，对牛骨骼肌的增殖和分化起着关键作用。
