[苏云金芽孢杆菌萌发孢子中氨基酸、矿质元素含量及各种酶的活性]

[Amino acid and mineral element content and the activity of various enzymes in germinating spores of Bacillus thuringiensis].

作者信息

Alekseev A N, Karabanova L N, Kraĭnova O A, Krasov E S, Kashparova E V

出版信息

Mikrobiologiia. 1985 Mar-Apr;54(2):181-5.

Abstract

Changes in the content of dipicolinic acid and mineral elements were studied in the process of Bacillus thuringiensis spore germination. The spores released up to 28% of dipicolinic acid and 18% of calcium at the activation stage, and 93 and 91%, respectively, at the initiation stage. At the same time, the content of Mg, Mn, Zn and P decreased while K, Na and Fe accumulated in the spores. The activities of total and serine proteases, alkaline phosphatase, NADH dehydrogenase and aldolase increased in the extract of initiated spores. The content of glutamate decreased in the free amino acid pool as early as by the 30th second of the initiation stage.

摘要

研究了苏云金芽孢杆菌孢子萌发过程中吡啶二羧酸和矿质元素含量的变化。在激活阶段，孢子释放出高达28%的吡啶二羧酸和18%的钙，而在萌发起始阶段分别释放出93%和91%。同时，孢子中镁、锰、锌和磷的含量降低，而钾、钠和铁则积累。萌发起始孢子提取物中总蛋白酶、丝氨酸蛋白酶、碱性磷酸酶、NADH脱氢酶和醛缩酶的活性增加。早在萌发起始阶段的第30秒，游离氨基酸池中谷氨酸的含量就开始下降。