菌株 TR-4 防治 × Dress 叶部病害的作用机制

Mechanism of strain TR-4 against leaf disease of × Dress.

机构信息

Nanjing Forestry University, Nanjing, Jiangsu, China.

Jinling Institute of Technology, Nanjing, Jiangsu, China.

出版信息

PeerJ. 2024 Jun 25;12:e17568. doi: 10.7717/peerj.17568. eCollection 2024.

DOI:10.7717/peerj.17568

PMID:38948232

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11212632/

Abstract

BACKGROUND

species are among the most common pathogens in agriculture and forestry, and their control is urgently needed.

METHODS

In this study, a total of 68 strains of biocontrol bacteria were isolated and identified from × rhizosphere soil.

RESULTS

The isolates were identified as by 16S rRNA. The inhibitory effect of TR-4 on was confirmed by an antagonistic experiment. The inhibitory effect of TR-4 was 98% at a concentration of 10 µl/ml bacterial solution, protection of the plant and inhibition of was evident. Moreover, the secretion of cellulase and chitosan enzymes in the TR-4 fermentation liquid cultured for three days was 9.07 mol/L and 2.15 µl/mol, respectively. Scanning electron microscopy and transmission electron microscopy confirmed that TR-4 destroyed the cell wall of , resulting in leakage of the cell contents, thus weakening the pathogenicity of the bacteria.

摘要

背景

是农业和林业中最常见的病原体之一，因此急需对其进行控制。

方法

本研究从 × 的根际土壤中分离并鉴定了 68 株生防细菌。

结果

通过 16S rRNA 鉴定这些分离株为。通过拮抗实验证实了 TR-4 对的抑制作用。当细菌溶液浓度为 10 µl/ml 时，TR-4 的抑制率为 98%，对植物的保护和对的抑制作用明显。此外，在培养 3 天的 TR-4 发酵液中，纤维素酶和壳聚糖酶的分泌量分别为 9.07 mol/L 和 2.15 µl/mol。扫描电子显微镜和透射电子显微镜证实，TR-4 破坏了的细胞壁，导致细胞内容物泄漏，从而削弱了细菌的致病性。