Lee Tong Geon
Horticultural Sciences Department, University of Florida, Gainesville, FL, USA.
Data Brief. 2024 May 29;55:110567. doi: 10.1016/j.dib.2024.110567. eCollection 2024 Aug.
The large-fruited fresh-market tomato cultivated in the U.S. represents a unique fruit market class of contemporary (modern) tomatoes for direct consumption. The genomes of F plants from crosses between inbred contemporary U.S. large-fruited fresh-market tomatoes were sequenced. 516 F individual plants randomly selected from five different biparental segregating populations were used for DNA extraction. The polymerase chain reaction (PCR)-free, paired-end (2 × 150 bp) sequencing libraries (350 bp DNA fragment length) were prepared, and sequenced on average 5 Gb for each plant using the Illumina next-generation sequencing technologies [1,2]. Raw Illumina reads with adapter contamination and/or uncertain nucleotides constitute (Ns, >10 % of either read; Q-score 5 or lower, >50 % of either read) were removed. This data article will contribute to improving our knowledge of the genetic recombination and variation in tomato.
在美国种植的大果型鲜食番茄代表了当代(现代)用于直接消费的独特水果市场类别番茄。对美国当代自交系大果型鲜食番茄杂交产生的F植株的基因组进行了测序。从五个不同的双亲分离群体中随机选择516株F单株用于DNA提取。制备了无聚合酶链反应(PCR)、双端(2×150 bp)测序文库(DNA片段长度350 bp),并使用Illumina下一代测序技术对每株植物平均测序5 Gb [1,2]。去除了带有接头污染和/或不确定核苷酸(Ns,任一读数的>10%;Q分数为5或更低,任一读数的>50%)的原始Illumina读数。这篇数据文章将有助于提高我们对番茄基因重组和变异的认识。