Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi County, Taiwan; Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.
Department of Medical Research, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
J Environ Manage. 2024 Aug;366:121688. doi: 10.1016/j.jenvman.2024.121688. Epub 2024 Jul 5.
Heavy metals (HMs) contained terrestrial ecosystems are often significantly display the antibiotic resistome in the pristine area due to increasing pressure from anthropogenic activity, is complex and emerging research interest. This study investigated that impact of chromium (Cr), nickel (Ni), cobalt (Co) concentrations in serpentine soil on the induction of antibiotic resistance genes and antimicrobial resistance within the native bacterial community as well as demonstrated their metabolic fingerprint. The full-length 16S-rRNA amplicon sequencing observed an increased abundance of Firmicutes, Actinobacteriota, and Acidobacteriota in serpentine soil. The microbial community in serpentine soil displayed varying preferences for different carbon sources, with some, such as carbohydrates and carboxylic acids, being consistently favored. Notably, 27 potential antibiotic resistance opportunistic bacterial genera have been identified in different serpentine soils. Among these, Lapillicoccus, Rubrobacter, Lacibacter, Chloroplast, Nitrospira, Rokubacteriales, Acinetobacter, Pseudomonas were significantly enriched in high and medium HMs concentrated serpentine soil samples. Functional profiling results illustrated that vancomycin resistance pathways were prevalent across all groups. Additionally, beta-lactamase, aminoglycoside, tetracycline, and vancomycin resistance involving specific bio-maker genes (ampC, penP, OXA, aacA, strB, hyg, aph, tet(A/B), otr(C), tet(M/O/Q), van(A/B/D), and vanJ) were the most abundant and enriched in the HMs-contaminated serpentine soil. Overall, this study highlighted that heavy-metal enriched serpentine soil is potential to support the proliferation of bacterial antibiotic resistance in native microbiome, and might able to spread antibiotic resistance to surrounding environment.
重金属（HMs）在陆地生态系统中普遍存在，由于人为活动的压力不断增加，其在原始地区的抗生素抗性基因库也经常显著显示出来，这是一个复杂且新兴的研究热点。本研究调查了蛇纹石土壤中铬（Cr）、镍（Ni）、钴（Co）浓度对土著细菌群落中抗生素抗性基因的诱导以及对其代谢指纹图谱的影响。全长 16S-rRNA 扩增子测序观察到蛇纹石土壤中厚壁菌门、放线菌门和酸杆菌门的丰度增加。蛇纹石土壤中的微生物群落对不同碳源表现出不同的偏好，一些碳源，如碳水化合物和羧酸，始终受到青睐。值得注意的是，在不同的蛇纹石土壤中发现了 27 种潜在的抗生素耐药机会细菌属。其中，Lapillicoccus、Rubrobacter、Lacibacter、Chloroplast、Nitrospira、Rokubacteriales、Acinetobacter、Pseudomonas 在高浓度和中浓度重金属集中的蛇纹石土壤样本中显著富集。功能分析结果表明，万古霉素抗性途径在所有组中都很普遍。此外，β-内酰胺酶、氨基糖苷类、四环素和万古霉素抗性涉及特定的生物标志物基因（ampC、penP、OXA、aacA、strB、hyg、aph、tet(A/B)、otr(C)、tet(M/O/Q)、van(A/B/D)和 vanJ）在受重金属污染的蛇纹石土壤中最为丰富和富集。总体而言，本研究强调了富含重金属的蛇纹石土壤有可能支持土著微生物群落中细菌抗生素抗性的增殖，并可能将抗生素抗性传播到周围环境。
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