Jiang Shi-Shi, Nie Hong-Bing, Hua Shan, Xie Meng, Xu Ren-Shi
Department of Neurology, Jiangxi Provincial People's Hospital, Clinical College of Nanchang Medical College, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, Jiangxi, China.
Department of Ultrasonography, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, Jiangxi, China.
Curr Protein Pept Sci. 2025;26(1):57-75. doi: 10.2174/0113892037293525240621120033.
Proteomic elucidation is an essential step in improving our understanding of the biological properties of proteins in amyotrophic lateral sclerosis (ALS).
Preliminary proteomic analysis was performed on the spinal cord and brain of SOD1 G93A (TG) and wild-type (WT) mice using isobaric tags for relative and absolute quantitation.
Partial up- and downregulated proteins showing significant differences between TG and WT mice were identified, of which 105 proteins overlapped with differentially expressed proteins in both the spinal cord and brain of progression mice. Bioinformatic analyses using Gene Ontology, a cluster of orthologous groups, and Kyoto Encyclopedia of Genes and Genomes pathway revealed that the significantly up- and downregulated proteins represented multiple biological functions closely related to ALS, with 105 overlapping differentially expressed proteins in the spinal cord and brain at the progression stage of TG mice closely related to 122 pathways. Differentially expressed proteins involved in a set of molecular functions play essential roles in maintaining neural cell survival.
This study provides additional proteomic profiles of TG mice, including potential overlapping proteins in both the spinal cord and brain that participate in pathogenesis, as well as novel insights into the up- and downregulation of proteins involved in the pathogenesis of ALS.
蛋白质组学解析是增进我们对肌萎缩侧索硬化症（ALS）中蛋白质生物学特性理解的关键步骤。
使用相对和绝对定量的等压标签，对SOD1 G93A（转基因，TG）小鼠和野生型（WT）小鼠的脊髓和脑进行初步蛋白质组学分析。
鉴定出了TG小鼠和WT小鼠之间部分上调和下调且存在显著差异的蛋白质，其中105种蛋白质与疾病进展期小鼠脊髓和脑中差异表达的蛋白质重叠。使用基因本体论、直系同源簇和京都基因与基因组百科全书通路进行的生物信息学分析表明，显著上调和下调的蛋白质代表了与ALS密切相关的多种生物学功能，TG小鼠疾病进展期脊髓和脑中105种重叠的差异表达蛋白质与122条通路密切相关。参与一组分子功能的差异表达蛋白质在维持神经细胞存活中起重要作用。
本研究提供了TG小鼠额外的蛋白质组学概况，包括脊髓和脑中可能参与发病机制的重叠蛋白质，以及对ALS发病机制中蛋白质上调和下调的新见解。
