MpANT 调控卷柏的分生组织发育。

MpANT regulates meristem development in Marchantia polymorpha.

机构信息

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China; Key Laboratory of Plant Carbon Capture, CAS, Shanghai 200032, China; Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands.

出版信息

Cell Rep. 2024 Jul 23;43(7):114466. doi: 10.1016/j.celrep.2024.114466. Epub 2024 Jul 9.

DOI:10.1016/j.celrep.2024.114466

PMID:38985681

Abstract

Meristems are crucial for organ formation, but our knowledge of their molecular evolution is limited. Here, we show that AINTEGUMENTA (MpANT) in the euANT branch of the APETALA2-like transcription factor family is essential for meristem development in the nonvascular plant Marchantia polymorpha. MpANT is expressed in the thallus meristem. Mpant mutants show defects to maintain meristem identity and undergo meristem duplication, while MpANT overexpressers show ectopic thallus growth. MpANT directly upregulates MpGRAS9 in the SHORT-ROOT (SHR) branch of the GRAS family. In the vascular plant Arabidopsis thaliana, the euANT-branch genes PLETHORAs (AtPLTs) and AtANT are involved in the formation and maintenance of root/shoot apical meristems and lateral organ primordia, and AtPLTs directly target SHR-branch genes. In addition, euANTs bind through a similar DNA-binding motif to many conserved homologous genes in M. polymorpha and A. thaliana. Overall, the euANT pathway has an evolutionarily conserved role in meristem development.

摘要

分生组织对于器官形成至关重要，但我们对其分子进化的了解有限。在这里，我们表明，APETALA2 样转录因子家族 euANT 分支中的 AINTEGUMENTA（MpANT）对于非维管束植物 Marchantia polymorpha 中的分生组织发育是必需的。MpANT 在叶状体分生组织中表达。Mpant 突变体显示出维持分生组织身份的缺陷，并经历分生组织复制，而 MpANT 过表达体表现出异位叶状体生长。MpANT 直接上调 GRAS 家族 SHR 分支中的 MpGRAS9。在维管植物拟南芥中，euANT 分支基因 PLETHORAs（AtPLTs）和 AtANT 参与根/茎顶端分生组织和侧生器官原基的形成和维持，并且 AtPLTs 直接靶向 SHR 分支基因。此外，euANT 通过类似的 DNA 结合基序与 M. polymorpha 和 A. thaliana 中的许多保守同源基因结合。总体而言，euANT 途径在分生组织发育中具有进化保守的作用。