胎牛血清纤连蛋白和内源性大鼠细胞纤连蛋白在细胞外基质中的分布。
Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix.
作者信息
Hayman E G, Ruoslahti E
出版信息
J Cell Biol. 1979 Oct;83(1):255-9. doi: 10.1083/jcb.83.1.255.
Abstract
Normal rat kidney cells were cultured in medium supplemented with normal fetal bovine serum (FBS) or FBS depleted of fibronectin. The cell surface fibronectin of these cultures was visualized by indirect immunofluorescence using species-specific antisera for either rat fibronectin or bovine fibronectin. Anti-rat-fibronectin revealed fibrillar structures on the cells grown in either normal medium or fibronectin-depleted medium. Anti-bovine fibronectin revealed similar fibrillar networks, but only on the cells grown in medium containing bovine fibronectin. Staining in each case was abolished by absorption with the homologous antigen. It appears that exogenous fibronectin was incorporated into the same structures as endogenous fibronectin. This finding suggests that circulating fibronectin may serve as a building block for the assembly of extracellular matrix, possibly by cells which are incapable of synthesizing it.
摘要
将正常大鼠肾细胞培养于添加了正常胎牛血清（FBS）或去除纤连蛋白的FBS的培养基中。使用针对大鼠纤连蛋白或牛纤连蛋白的种属特异性抗血清，通过间接免疫荧光观察这些培养物的细胞表面纤连蛋白。抗大鼠纤连蛋白在正常培养基或去除纤连蛋白的培养基中生长的细胞上显示出纤维状结构。抗牛纤连蛋白显示出类似的纤维状网络，但仅在含有牛纤连蛋白的培养基中生长的细胞上。在每种情况下，染色都被同源抗原吸收所消除。似乎外源性纤连蛋白与内源性纤连蛋白整合到相同的结构中。这一发现表明，循环中的纤连蛋白可能作为细胞外基质组装的构建块，可能由无法合成它的细胞来完成。
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