Department of Horticulture, University of Georgia, Athens, GA 30602, USA.
Department of Statistics, University of Georgia, Athens, GA 30602, USA.
Sensors (Basel). 2024 Jun 29;24(13):4228. doi: 10.3390/s24134228.
Salinity stress is a common challenge in plant growth, impacting seed quality, germination, and general plant health. Sodium chloride (NaCl) ions disrupt membranes, causing ion leakage and reducing seed viability. Gibberellic acid (GA) treatments have been found to promote germination and mitigate salinity stress on germination and plant growth. 'Bauer' and 'Muir' lettuce () seeds were soaked in distilled water (control), 100 mM NaCl, 100 mM NaCl + 50 mg/L GA, and 100 mM NaCl + 150 mg/L GA in Petri dishes and kept in a dark growth chamber at 25 °C for 24 h. After germination, seedlings were monitored using embedded cameras, capturing red, green, and blue (RGB) images from seeding to final harvest. Despite consistent germination rates, 'Bauer' seeds treated with NaCl showed reduced germination. Surprisingly, the 'Muir' cultivar's final dry weight differed across treatments, with the NaCl and high GA concentration combination yielding the poorest results ( < 0.05). This study highlights the efficacy of GA applications in improving germination rates. However, at elevated concentrations, it induced excessive hypocotyl elongation and pale seedlings, posing challenges for two-dimensional imaging. Nonetheless, a sigmoidal regression model using projected canopy size accurately predicted dry weight across growth stages and cultivars, emphasizing its reliability despite treatment variations ( = 0.96, = 0.11, < 0.001).
盐胁迫是植物生长过程中的常见挑战，影响种子质量、萌发和整体植物健康。氯化钠 (NaCl) 离子破坏细胞膜，导致离子泄漏，降低种子活力。已发现赤霉素 (GA) 处理可促进萌发，并减轻盐胁迫对萌发和植物生长的影响。将“Bauer”和“Muir”生菜种子浸泡在蒸馏水中（对照）、100 mM NaCl、100 mM NaCl + 50 mg/L GA 和 100 mM NaCl + 150 mg/L GA 的 Petri 盘中，并在 25°C 的黑暗生长室中保持 24 小时。萌发后，使用嵌入式摄像头监测幼苗，从播种到最终收获捕获红、绿、蓝 (RGB) 图像。尽管萌发率一致，但用 NaCl 处理的“Bauer”种子的萌发率降低。令人惊讶的是，“Muir”品种的最终干重因处理而异，NaCl 和高 GA 浓度组合的结果最差（<0.05）。本研究强调了 GA 应用在提高萌发率方面的功效。然而，在高浓度下，它会诱导下胚轴过度伸长和幼苗苍白，这对二维成像构成挑战。尽管存在处理差异，但使用投影冠层大小的 sigmoidal 回归模型准确预测了整个生长阶段和品种的干重（=0.96，=0.11，<0.001），强调了其可靠性。
