UPLC-LTQ-Orbitrap 法研究 5-甲氧基-α-甲基色胺在大鼠尿中的代谢产物。
UPLC-LTQ-Orbitrap Study on Rat Urinary Metabolites of 5-Methoxy-Alpha-Methyltryptamine.
机构信息
Department of Toxicants and Narcotics Examination Office, Shanghai Municipal Public Security Bureau, Shanghai, 200083, PR China.
School of Pharmacy, Fudan University, Department of Toxicants & Navcotics Examination Office, Shanghai, 201203, PR China.
出版信息
Curr Drug Metab. 2024;25(4):298-305. doi: 10.2174/0113892002295551240628061732.
OBJECTIVE
5-Methoxy-α-Methyltryptamine (5-MeO-AMT) is a new psychoactive substance which is abused due to its hallucinogenic and euphoric effects. This study aimed to study the metabolic characteristics of 5-MeO-AMT.
METHODS
Five rats were given intraperitoneal injection at a dose of 50 mg/kg of 5-MeO-AMT, and their urine was subsequently collected at different times within 7 days. Ultra-high performance liquid chromatographytandem high-resolution mass spectrometry (UPLC-LTQ-Orbitrap) was used to detect the precise molecular weight and fragment ions of 5-MeO-AMT and its possible metabolites in the urine sample extracted with benzene-ethyl acetate.
RESULTS
Three metabolites, including OH-5-MeO-AMT, α-Me-5-HT, and N-Acetyl-5-MeO-AMT were identified in rats' urine. The major metabolic pathways involved O-demethylation, hydroxylation of indole ring, and Acetylation on aliphatic amines.
CONCLUSION
The results of this study are an important reference for the identification and screening of toxicants of 5-MeO-AMT.
目的
5-甲氧基-α-甲基色胺（5-MeO-AMT）是一种新的精神活性物质，由于其致幻和欣快的作用而被滥用。本研究旨在研究 5-MeO-AMT 的代谢特征。
方法
5 只大鼠按 50mg/kg 的剂量腹腔注射 5-MeO-AMT，在 7 天内的不同时间收集尿液。采用超高效液相色谱-串联高分辨率质谱（UPLC-LTQ-Orbitrap）法检测苯-乙酸乙酯提取尿液样品中 5-MeO-AMT 及其可能代谢物的精确分子量和碎片离子。
结果
在大鼠尿液中鉴定出 3 种代谢物，包括 OH-5-MeO-AMT、α-Me-5-HT 和 N-乙酰-5-MeO-AMT。主要代谢途径涉及 O-去甲基化、吲哚环羟化和脂肪族胺的乙酰化。
结论
本研究结果为 5-MeO-AMT 的毒物鉴定和筛选提供了重要参考。
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