一种用于快速检测病毒SC15的比色逆转录环介导等温扩增检测方法
A Colorimetric RT-LAMP Assay for Rapid Detection of Virus SC15.
作者信息
Li Chen, Guo Shuixian, Sun Min, Niu Jingping, Yin Congcong, Du Weijun, Zhao Jinzhong, Liu Dingbin, Yue Aiqin
机构信息
Department of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
出版信息
ACS Omega. 2024 Jun 24;9(27):29765-29775. doi: 10.1021/acsomega.4c03372. eCollection 2024 Jul 9.
(SMV) represents one of the most devastating viral diseases affecting soybeans worldwide. Among its strains, SMV-SC15 is notable for its virulence, predominance, and widespread occurrence. Rapid and on-site diagnosis is important for controlling the spread of SMV-SC15. In this study, we proposed a colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SMV-SC15 using three color indicators for visual interpretation: Neutral Red (N-Red), Bromothymol Blue (BTB), and SYBR Green I. The SMV-SC15 in the soybean tissue was detected with remarkable sensitivity and specificity within 30 min, achieving a detection limit as low as 10 ng/μL. 200 soybean leaf samples from the field were analyzed by the colorimetric RT-LAMP assays, holding significant potential for rapid screening of SMV-SC15-resistant cultivars, thereby contributing to effective SMV control.
大豆花叶病毒（SMV）是全球范围内影响大豆的最具毁灭性的病毒性疾病之一。在其毒株中，SMV-SC15以其毒力、优势地位和广泛传播而引人注目。快速现场诊断对于控制SMV-SC15的传播至关重要。在本研究中，我们提出了一种比色逆转录环介导等温扩增（RT-LAMP）检测方法，用于检测SMV-SC15，该方法使用三种颜色指示剂进行视觉判读：中性红（N-Red）、溴百里酚蓝（BTB）和SYBR Green I。大豆组织中的SMV-SC15在30分钟内被检测出，具有显著的灵敏度和特异性，检测限低至10 ng/μL。通过比色RT-LAMP检测方法对200份田间大豆叶片样本进行了分析，该方法在快速筛选抗SMV-SC15品种方面具有巨大潜力，从而有助于有效控制SMV。
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