Department of Nutrition, School of Public Health, Guangdong Medical University, Dongguan 523808, China.
Department of Nutrition, School of Public Health, Guangdong Medical University, Dongguan 523808, China; Dongguan Key Laboratory of Prevention and Treatment of Chronic Noncommunicable Diseases, School of Public Health, Guangdong Medical University, Dongguan 523808, China.
Food Chem. 2024 Nov 30;459:140446. doi: 10.1016/j.foodchem.2024.140446. Epub 2024 Jul 14.
Hibiscus sabdariffa L. (roselle) is a medicinal and edible plant which rich in anthocyanins with potent antioxidant properties. To enhance the stability of roselle anthocyanins, they were encapsulated in nanocapsules composed of carboxymethyl chitosan (CMC), chitosan hydrochloride (CHC), and β-lactoglobulin (β-Lg). In vitro simulated digestion assays evaluated the impact of various core-to-wall ratios and β-Lg concentrations on the bioaccessibility of seven anthocyanins. Nanocapsules with a core-to-wall ratio of 1:2 and β-Lg at 10 mg/mL exhibited the highest encapsulation efficiency (EE). Cyanidin-3-glucoside had the highest EE, while cyanidin-3-sambubioside showed the outstanding retention rate. Furthermore, simulated digestion experiments combined with molecular docking revealed that peonidin-3-glucoside and petunidin-3-glucoside likely interact with and bind to the outer β-Lg layer of the nanocapsules, increasing their release during in vitro digestion. This study demonstrates that encapsulating roselle anthocyanins in CMC, CHC, and β-Lg nanocapsules significantly enhances their bioaccessibility.
玫瑰茄（洛神花）是一种药用和食用植物，富含具有强大抗氧化性能的花色苷。为了提高玫瑰茄花色苷的稳定性，将其包封在由羧甲基壳聚糖（CMC）、壳聚糖盐酸盐（CHC）和β-乳球蛋白（β-Lg）组成的纳米胶囊中。体外模拟消化试验评估了不同核壳比和β-Lg 浓度对七种花色苷生物利用度的影响。核壳比为 1:2 且β-Lg 浓度为 10mg/mL 的纳米胶囊表现出最高的包封效率（EE）。矢车菊素-3-葡萄糖苷具有最高的 EE，而矢车菊素-3-桑布糖苷表现出出色的保留率。此外，模拟消化实验结合分子对接表明，芍药素-3-葡萄糖苷和锦葵素-3-葡萄糖苷可能与纳米胶囊的外层β-Lg 层相互作用并结合，从而增加其在体外消化过程中的释放。本研究表明，将玫瑰茄花色苷包封在 CMC、CHC 和 β-Lg 纳米胶囊中可显著提高其生物利用度。
