Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R. China.
Guangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi, Guangxi Academy of Specialty Crops, Guilin, Guangxi 541004, P.R. China.
Plant Dis. 2024 Nov;108(11):3393-3399. doi: 10.1094/PDIS-12-23-2575-RE. Epub 2024 Nov 6.
Citrus chlorotic dwarf disease (CCDD) seriously affects the citrus industry. Citrus chlorotic dwarf-associated virus (CCDaV) is speculated to be the causal agent of CCDD. However, this speculation has not been confirmed by fulfilling Koch's postulates. In this study, an infectious clone comprising a 1.6-fold tandem CCDaV genome in the binary vector pBinPLUS was constructed and agro-inoculated into 'Eureka' lemon () seedlings through vacuum infiltration. At 60 days postinoculation, 25% of the 'Eureka' lemon seedlings developed symptoms of crinkling and curling that were the same as those associated with the wild-type virus. Western blotting and graft transmission assays confirmed that the infectious clone systemically infected 'Eureka' lemon seedlings. In addition, CCDaV can establish infection on three more species and one hybrid, although at different infection rates. These findings support that CCDaV is the primary causal agent of CCDD. The infectious CCDaV clone will allow further studies on the functions of viral proteins and molecular interactions of CCDaV with its hosts.
柑橘衰退病（CCDD）严重影响柑橘产业。柑橘衰退矮缩相关病毒（CCDaV）被推测是 CCDD 的致病因子。然而，这一推测尚未通过满足科赫假设得到证实。在本研究中，构建了一个包含双元载体 pBinPLUS 中 1.6 倍串联 CCDaV 基因组的感染性克隆，并通过真空渗透法将其接种到‘尤力克’柠檬（）幼苗中。接种后 60 天，25%的‘尤力克’柠檬幼苗出现卷曲和卷曲症状，与野生型病毒相关。Western blot 和嫁接传递试验证实，该感染性克隆系统地感染了‘尤力克’柠檬幼苗。此外，CCDaV 还可以在另外 3 个种和 1 个杂种上建立感染，但感染率不同。这些发现支持 CCDaV 是 CCDD 的主要致病因子。该传染性 CCDaV 克隆将允许进一步研究病毒蛋白的功能以及 CCDaV 与宿主的分子相互作用。
Zotero 插件