Aihetanmu Sainaiwaer, Liang Zhixuan, Zhang Xueling, Luo Baolong, Zhang Huimin, Huang Jian, Tian Fengwei, Sun Hailong, Ni Yongqing
School of Food Science and Technology, Shihezi University, Shihezi, China.
School of Food Science and Technology, Jiangnan University, Wuxi, China.
Front Microbiol. 2024 Jul 3;15:1399743. doi: 10.3389/fmicb.2024.1399743. eCollection 2024.
Little has been known about symbiotic relationships and host specificity for symbionts in the human gut microbiome so far. are a paragon of the symbiotic bacteria biota in the human gut. In this study, we characterized the population genetic structure of three bifidobacteria species from 58 healthy mother-infant pairs of three ethnic groups in China, geographically isolated, by Rep-PCR, multi-locus sequence analysis (MLSA), and carbohydrate utilization. One hundred strains tested were incorporated into 50 sequence types (STs), of which 29 STs, 17 STs, and 4 STs belong to subsp. , , and subsp. , respectively. The conspecific strains from the same mother-child pair were genetically very similar, supporting the vertical transmission of phylotypes from mother to offspring. In particular, results based on allele profiles and phylogeny showed that subsp. and exhibited considerable intraspecies genetic heterogeneity across three ethnic groups, and strains were clustered into ethnicity-specific lineages. Yet almost all strains of subsp. were incorporated into the same phylogenetic clade, regardless of ethnic origin. Our findings support the hypothesis of co-evolution between human gut symbionts and their respective populations, which is closely linked to the lifestyle of specific bacterial lineages. Hence, the natural and evolutionary history of species would be an additional consideration when selecting bifidobacterial strains for industrial and therapeutic applications.
迄今为止，人们对人类肠道微生物群中共生关系和共生菌的宿主特异性了解甚少。[具体菌种名称]是人类肠道中共生细菌生物群的典范。在本研究中，我们通过重复聚合酶链反应（Rep-PCR）、多位点序列分析（MLSA）和碳水化合物利用，对来自中国三个地理隔离的民族的58对健康母婴中的三种双歧杆菌的群体遗传结构进行了表征。测试的100株菌株被归入50个序列类型（STs），其中29个STs、17个STs和4个STs分别属于[具体亚种名称1]亚种、[具体亚种名称2]亚种和[具体亚种名称3]亚种。来自同一母婴对的同种菌株在遗传上非常相似，支持了[具体菌种名称]系统型从母亲到后代的垂直传播。特别是，基于等位基因谱和系统发育的结果表明，[具体亚种名称1]亚种和[具体亚种名称2]亚种在三个民族中表现出相当大的种内遗传异质性，并且菌株聚集成特定民族的谱系。然而，几乎所有的[具体亚种名称3]亚种菌株都被归入同一个系统发育分支，无论其种族来源如何。我们的研究结果支持了人类肠道共生菌与其各自群体共同进化的假设，这与特定细菌谱系的生活方式密切相关。因此，在选择用于工业和治疗应用的双歧杆菌菌株时，[具体菌种名称]的自然和进化历史将是一个额外的考虑因素。
