Akgül Davut Soner, Önder Serkan, Savaş Nurdan Güngör, Yıldız Murat, Bülbül İzzet, Özarslandan Mümine
Department of Plant Protection, Agriculture Faculty, Çukurova University, 01330 Adana, Türkiye.
Department of Plant Protection, Agriculture Faculty, Eskişehir Osmangazi University, 26160 Eskişehir, Türkiye.
J Fungi (Basel). 2024 Jun 24;10(7):444. doi: 10.3390/jof10070444.
species are agriculturally important fungi with a broad host range and can be found as endophytic, pathogenic, or opportunistic parasites in many crop plants. This study aimed to identify species in bare-rooted, dormant plants in Turkish grapevine nurseries using molecular identification methods and assess their pathogenicity. Asymptomatic dormant plants were sampled from grapevine nurseries (43) in different regions of the country, and fungi were isolated from plant roots and internal basal tissues. The strains were identified by performing gene sequencing (, ) and phylogenetic analyses. Pathogenicity tests were carried out by inoculating mycelial agar pieces of strains onto the stem or conidial suspensions into the rhizosphere of vines (1103 Paulsen rootstock). Laboratory tests revealed that species were highly prevalent in Turkish grapevine nurseries (41 out of 43). Gene sequencing and phylogenetic analyses unraveled that 12 species ( and spp.) existed in the ready-to-sale plants. Some of these species ( and ) consistently caused wood necrosis of seedling stems, rotting of the basal zone and roots, and reduced root biomass. Although the other nine species also caused some root rot and root reduction, their virulence was not as severe as the pathogenic ones, and they were considered opportunistic parasites or endophytic species. This study suggests that species might play an important role in root-basal rot, wood canker symptoms, and young vine decline in Turkish grapevine nurseries and that these species need to be considered for healthy seedling production.
该物种是具有广泛寄主范围的重要农业真菌，可在许多作物植物中作为内生菌、病原菌或机会性寄生虫被发现。本研究旨在利用分子鉴定方法鉴定土耳其葡萄苗圃中裸根休眠植物中的该物种，并评估其致病性。从该国不同地区的葡萄苗圃（43个）采集无症状休眠植物，从植物根部和内部基部组织分离真菌。通过进行基因测序（……）和系统发育分析来鉴定这些菌株。通过将菌株的菌丝琼脂块接种到茎上或将分生孢子悬浮液接种到葡萄藤（1103保尔森砧木）的根际进行致病性测试。实验室测试表明，该物种在土耳其葡萄苗圃中高度普遍（43个中有41个）。基因测序和系统发育分析表明，在待售植物中存在12种该物种（……和……属）。其中一些物种（……和……）持续导致幼苗茎干木质部坏死、基部区域和根部腐烂以及根生物量减少。虽然其他9种也导致了一些根腐和根量减少，但其毒力不如致病物种严重，它们被认为是机会性寄生虫或内生菌物种。本研究表明，该物种可能在土耳其葡萄苗圃的根基部腐烂、木材溃疡症状和幼龄葡萄衰退中起重要作用，并且在健康苗木生产中需要考虑这些物种。
