Liu Yinglong, Ahmed Ayesha, Munir Shahzad, Chen Lei, He Pengfei, He Yueqiu, Tang Ping, Kong Baohua, Wu Yixin, He Pengbo
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
College of Agronomy and Life Science, Zhaotong University, Zhaotong 657000, China.
J Fungi (Basel). 2024 Jul 17;10(7):494. doi: 10.3390/jof10070494.
Dodder ( spp.) is a dangerous parasitic plant that causes serious damage to crop production and is challenging to eliminate. Herbicide application is a common strategy to control dodder in the field, but it is costly, ineffective, and further results in hazardous outcomes. Therefore, our study aims to identify the potential pathogens in naturally occurring dodder infections which may provide efficient biocontrol options. In this regard, the pathogens were isolated from the infected plants, their pathogenicity was validated through inoculation, and the optimal culture conditions for their growth were identified by determining the pathogenicity difference. The pathogenicity range was determined in vitro using the leaves of common horticultural plants and crops. Furthermore, a small range of horticultural plants parasitized by in the field were inoculated with the pathogen to determine their biosafety and biocontrol potential, and the pathogens were identified by morphological and molecular characterization. We found 7 strains that were isolated after pathogen enrichment culture. Among them, Cbp6 and Cbp7 showed the highest pathogenicity against . After testing the inoculation of more than 50 species of plants, only 9 species showed varying degrees of lesions on leaves, which proved the high biosafety for common plants. Field spraying of these pathogens showed a good control effect on after 21 days; the disease severityreached 66.0%, while its host plant did not display obvious symptoms. In conclusion, the pathogens Cbp6 and Cbp7 were identified as , and the results of this study provide a theoretical basis for the biological control of dodder.
菟丝子（菟丝子属）是一种危险的寄生植物，会对作物生产造成严重损害，且难以根除。施用除草剂是田间控制菟丝子的常用策略，但成本高、效果不佳，还会带来有害后果。因此，我们的研究旨在确定自然发生的菟丝子感染中的潜在病原体，这可能提供有效的生物防治选择。在这方面，从受感染的植物中分离出病原体，通过接种验证其致病性，并通过确定致病性差异来确定其生长的最佳培养条件。使用常见园艺植物和作物的叶片在体外确定致病性范围。此外，对接种了田间寄生菟丝子的一小部分园艺植物接种病原体，以确定其生物安全性和生物防治潜力，并通过形态学和分子特征鉴定病原体。我们发现经过病原体富集培养后分离出7个菌株。其中，Cbp6和Cbp7对菟丝子表现出最高的致病性。在对50多种植物进行接种测试后，只有9种植物的叶片出现了不同程度的病斑，这证明了对常见植物具有较高的生物安全性。对这些病原体进行田间喷雾处理，21天后对菟丝子显示出良好的防治效果；病情严重程度达到66.0%，而其寄主植物未表现出明显症状。总之，病原体Cbp6和Cbp7被鉴定为[具体名称未明确]，本研究结果为菟丝子的生物防治提供了理论依据。
