Yin Xiangtian, Chen Zihe, Li Tinggang, Liu Qibao, Jiang Xilong, Han Xing, Wang Chundong, Wei Yanfeng, Yuan Lifang
Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, China.
J Fungi (Basel). 2024 Jul 22;10(7):508. doi: 10.3390/jof10070508.
is a dominant phytopathogen of grape white rot in China, significantly impacting grape yield and quality. Previous studies showed that the growth and pathogenicity of were affected by the environmental pH. Arrestin-like protein PalF plays a key role in mediating the activation of an intracellular-signaling cascade in response to alkaline ambient. However, it remains unclear whether palF affects the growth, development, and virulence of during the sensing of environmental pH changes. In this study, we identified a homologous gene of PalF/Rim8 in and constructed -silenced strains via RNA interference. -silenced strains exhibited impaired fungal growth at neutral/alkaline pH, accompanied by reduced pathogenicity compared to the wild-type (WT) and empty vector control (CK) strains. The distance between the hyphal branches was significantly increased in the -silenced strains. Additionally, -silenced strains showed increased sensitivity to NaCl, HO, and Congo red, and decreased sensitive to CaSO. RT-qPCR analysis demonstrated that the expression level of genes related to pectinase and cellulase were significantly down-regulated in -silenced strains compared to WT and CK strains. Moreover, the expression of , was directly or indirectly affected by silencing . Additionally, the expression of genes related to plant cell wall-degrading enzymes, which are key virulence factors for plant pathogenic fungi, was regulated by . Our results indicate the important roles of in growth, osmotolerance, and pathogenicity in .
是中国葡萄白腐病的主要植物病原菌，对葡萄产量和品质有重大影响。先前的研究表明，其生长和致病性受环境pH值的影响。类 Arrestin 蛋白 PalF 在介导细胞内信号级联反应以响应碱性环境中起关键作用。然而，尚不清楚 palF 在感知环境pH值变化过程中是否影响其生长、发育和毒力。在本研究中，我们鉴定了其 PalF/Rim8 的同源基因，并通过RNA干扰构建了沉默菌株。与野生型（WT）和空载体对照（CK）菌株相比，沉默菌株在中性/碱性pH条件下真菌生长受损，致病性降低。沉默菌株中菌丝分支之间的距离显著增加。此外，沉默菌株对NaCl、H₂O₂和刚果红的敏感性增加，对CaSO₄的敏感性降低。RT-qPCR分析表明，与果胶酶和纤维素酶相关的基因在沉默菌株中的表达水平与WT和CK菌株相比显著下调。此外，沉默直接或间接影响了某些基因的表达。此外，与植物细胞壁降解酶相关的基因（植物病原真菌的关键毒力因子）的表达受其调控。我们的结果表明其在生长、渗透耐受性和致病性方面的重要作用。
