Huang Linjie, Deng Jingwen, Su Yina, Hu Xueqi, Zhang Yichao, Hong Shanni, Lin Xiahui
School of Medical Imaging, Fujian Medical University, Fuzhou 350122, China.
Department of Otorhinolaryngology, Fujian Medical University Union Hospital, Fuzhou 350001, China.
Antioxidants (Basel). 2024 Jul 17;13(7):857. doi: 10.3390/antiox13070857.
Skin damage is one of the most prevalent human injuries, which affects the health of human beings. However, skin damage is often accompanied by bacterial infection and wound microenvironment changes, causing damage to normal cells and inhibiting wound healing. Herein, we designed a thermal-responsive antibacterial hydrogel (GAG hydrogel) loaded with catalase (CAT)-like Au@Pt@MgSiO nanoparticles (APM NPs) and gentamicin (GM) to promote wound healing. The GAG hydrogel was used in a photothermal therapy (PTT)/antibiotic combination to kill bacteria, reduce the use of antibiotics, improve the wound microenvironment, promote cell proliferation, and accelerate wound healing. Under near-infrared laser irradiation, APM NPs in the hydrogel generated local hyperthermia to kill bacteria. Meanwhile, the generated heat led to a change in the hydrogel's morphology, enabling it to release GM and APM NPs to prevent the overuse of antibiotics. Subsequently, the CAT-like ability of the APM NPs decreased the oxidative stress caused by hydrogen peroxide (HO), thus remodeling the wound microenvironment. Then, the weakly acidic microenvironment of the wound caused the decomposition of the APM NPs and the release of magnesium ions (Mg), promoting the growth and migration of cells for wound healing. Therefore, the studied thermal-responsive antibacterial (GAG) hydrogel has potential in the field of wound healing.
皮肤损伤是最常见的人类损伤之一，会影响人类健康。然而，皮肤损伤常伴有细菌感染和伤口微环境变化，对正常细胞造成损害并抑制伤口愈合。在此，我们设计了一种负载过氧化氢酶（CAT）样金@铂@硅酸镁纳米颗粒（APM NPs）和庆大霉素（GM）的热响应抗菌水凝胶（GAG水凝胶）以促进伤口愈合。GAG水凝胶用于光热疗法（PTT）/抗生素联合治疗，以杀死细菌、减少抗生素使用、改善伤口微环境、促进细胞增殖并加速伤口愈合。在近红外激光照射下，水凝胶中的APM NPs产生局部高温以杀死细菌。同时，产生的热量导致水凝胶形态发生变化，使其释放GM和APM NPs，防止抗生素过度使用。随后，APM NPs的类CAT能力降低了过氧化氢（HO）引起的氧化应激，从而重塑伤口微环境。然后，伤口的弱酸性微环境导致APM NPs分解并释放镁离子（Mg），促进细胞生长和迁移以实现伤口愈合。因此，所研究的热响应抗菌（GAG）水凝胶在伤口愈合领域具有潜力。
