Key Laboratory of National Forestry and Grassland Administration on Pest Chemical Control and Innovation Center of Pesticide Research, College of Science, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Int J Mol Sci. 2024 Jul 17;25(14):7830. doi: 10.3390/ijms25147830.
Bio-stimulants, such as selenium nanoparticles and melatonin, regulate melon growth. However, the effects of individual and combined applications of selenium nanoparticles and melatonin on the growth of melon seedlings have not been reported. Here, two melon cultivars were sprayed with selenium nanoparticles, melatonin, and a combined treatment, and physiological and biochemical properties were analyzed. The independent applications of selenium nanoparticles, melatonin, and their combination had no significant effects on the plant heights and stem diameters of Jiashi and Huangmengcui melons. Compared with the controls, both selenium nanoparticle and melatonin treatments increased soluble sugars (6-63%) and sucrose (11-88%) levels, as well as the activity of sucrose phosphate synthase (171-237%) in melon leaves. The phenylalanine ammonia lyase (29-95%), trans cinnamate 4-hydroxylase (32-100%), and 4-coumaric acid CoA ligase (26-113%), as well as mRNA levels, also increased in the phenylpropanoid metabolism pathway. Combining the selenium nanoparticles and melatonin was more effective than either of the single treatments. In addition, the levels of superoxide dismutase (43-130%), catalase (14-43%), ascorbate peroxidase (44-79%), peroxidase (25-149%), and mRNA in melon leaves treated with combined selenium nanoparticles and melatonin were higher than in controls. The results contribute to our understanding of selenium nanoparticles and melatonin as bio-stimulants that improve the melon seedlings' growth by regulating carbohydrate, polyamine, and antioxidant capacities.
生物刺激剂，如硒纳米粒子和褪黑素，可调节瓜类生长。然而，单独和联合应用硒纳米粒子和褪黑素对瓜苗生长的影响尚未报道。在这里，我们用硒纳米粒子、褪黑素和联合处理两种甜瓜品种，并分析了它们的生理生化特性。单独应用硒纳米粒子、褪黑素及其组合对伽师瓜和黄金蜜瓜的株高和茎粗没有显著影响。与对照相比，硒纳米粒子和褪黑素处理都增加了甜瓜叶片中的可溶性糖（6-63%）和蔗糖（11-88%）水平，以及蔗糖磷酸合成酶的活性（171-237%）。苯丙氨酸解氨酶（29-95%）、反式肉桂酸 4-羟化酶（32-100%）和 4-香豆酸辅酶 A 连接酶（26-113%）以及苯丙烷代谢途径中的 mRNA 水平也增加了。硒纳米粒子和褪黑素联合处理比单独处理更有效。此外，与对照相比，联合处理的甜瓜叶片中超氧化物歧化酶（43-130%）、过氧化氢酶（14-43%）、抗坏血酸过氧化物酶（44-79%）、过氧化物酶（25-149%）和 mRNA 的水平也更高。这些结果有助于我们理解硒纳米粒子和褪黑素作为生物刺激剂，通过调节碳水化合物、多胺和抗氧化能力来改善瓜苗的生长。
