梅毒螺旋体在体外暴露于亚杀菌剂量强力霉素下未产生耐药性：对强力霉素暴露后预防的启示。
In Vitro Exposure of Treponema pallidum to Subbactericidal Doxycycline Did Not Induce Resistance: Implications for Doxycycline Postexposure Prophylaxis.
作者信息
Tantalo Lauren C, Luetkemeyer Anne F, Lieberman Nicole A P, Nunley B Ethan, Avendaño Carlos, Greninger Alexander L, Celum Connie, Giacani Lorenzo
机构信息
Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA.
Division of HIV, Infectious Diseases, and Global Medicine, University of California, San Francisco, San Francisco, California, USA.
出版信息
J Infect Dis. 2025 Mar 17;231(3):729-733. doi: 10.1093/infdis/jiae381.
Doxycycline postexposure prophylaxis (doxy-PEP) could significantly reduce syphilis incidence. However, the increase in intermittent doxycycline usage might select resistant Treponema pallidum strains. To assess whether resistance to doxycycline could be induced in this pathogen, we exposed the SS14 strain in vitro, both intermittently and continuously, to a subbactericidal doxycycline concentration that still exerts antibiotic pressure. During and after each exposure experiment, we assessed the doxycycline minimal inhibitory concentration in test and control treponemes and performed whole-genome sequencing, concluding that no resistance developed. This work suggests that doxycycline-resistant T. pallidum is not an immediate threat for doxy-PEP implementation.
多西环素暴露后预防（多西环素-PEP）可显著降低梅毒发病率。然而，间歇性使用多西环素的增加可能会筛选出对梅毒螺旋体有抗性的菌株。为评估在该病原体中是否可诱导产生对多西环素的抗性，我们在体外将SS14菌株间歇性和持续性地暴露于仍施加抗生素压力的亚杀菌多西环素浓度下。在每次暴露实验期间及之后，我们评估了测试和对照梅毒螺旋体中的多西环素最小抑菌浓度，并进行了全基因组测序，得出未产生抗性的结论。这项研究表明，耐多西环素的梅毒螺旋体对多西环素-PEP的实施并非直接威胁。
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