Nutrition Research Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh.
Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Food Nutr Bull. 2024 Jun;45(2-3):67-73. doi: 10.1177/03795721241250104. Epub 2024 Jul 30.
A randomized controlled trial in Bangladeshi children aged 12 to 18 months with moderate acute malnutrition found that dietary supplementation with the microbiota-directed complementary food (MDCF-2) significantly improved weight gain and repaired gut microbiota compared to the ready-to-use supplementary food. However, the MDCF-2 formulation was made daily from locally available ingredients and the need for a packaged, nutritionally compliant, and organoleptically acceptable MDCF-2 prototype was essential for future large-scale clinical studies.
The study aimed to develop and assess the acceptability of 3 alternative foil-packaged formulations of MDCF-2 in comparison to current MDCF-2.
Of the 3 packaged formulations, the Jumpstart version was provided in 2 sachets, the other 2 formulations were provided in a retort-stable foil pouch extended by sterilization, and microbiological growth was monitored over 10 months. The acceptability study included 40 children aged 8 to 12 months living in an urban slum in Dhaka, and the organoleptic properties were assessed using a 7-point hedonic scale.
In the 100 g distributed over the 2 sessions, children consumed 82.5 ± 7.84 g (mean ± SD) of kitchen-prepared MDCF-2, 85.4 ± 7.15 g of the "Jumpstart" MDCF-2 formulation, 85.4 ± 8.70 g of the MDCF-2 with green banana powder, and 86.2 ± 4.26 g of the MDCF-2 with sweet potato formulation. The "Jumpstart" MDCF-2 and MDCF-2 with sweet potato achieved the highest overall acceptability scores on the hedonic scale; although none of the shelf-stable formulations were significantly different from the kitchen-prepared MDCF-2.
Packaged, shelf-stable prototypes of MDCF-2 exhibited comparable acceptability among Bangladeshi children aged 8 to 12 months to the original freshly prepared formulation.
Development and Acceptability of Shelf-Stable Microbiota-Directed Complementary Foods.
一项在 12 至 18 个月大的中度急性营养不良孟加拉国儿童中进行的随机对照试验发现，与即食补充食品相比，食用以微生物组为导向的补充食品（MDCF-2）可显著改善体重增加和肠道微生物群的恢复。然而，MDCF-2 的配方是由当地可用的食材每天制作的，因此需要一种包装的、符合营养要求且感官可接受的 MDCF-2 原型，这对于未来的大规模临床研究至关重要。
本研究旨在开发和评估 3 种替代箔包装 MDCF-2 配方与当前 MDCF-2 的可接受性。
在这 3 种包装配方中，Jumpstart 版本有 2 个小袋，其他 2 个配方在经过延长灭菌的箔袋中提供，并且在 10 个月的时间里监测微生物生长情况。接受性研究包括 40 名居住在达卡市区贫民窟的 8 至 12 个月大的儿童，感官特性使用 7 分喜好量表进行评估。
在 2 次分配的 100 克中，儿童分别消耗了 82.5 ± 7.84 克（平均值 ± SD）的厨房制备的 MDCF-2、85.4 ± 7.15 克的 Jumpstart MDCF-2 配方、85.4 ± 8.70 克的添加绿香蕉粉的 MDCF-2 配方和 86.2 ± 4.26 克的添加甘薯配方的 MDCF-2。Jumpstart MDCF-2 和添加甘薯的 MDCF-2 在喜好量表上获得了最高的总体可接受性评分；尽管这些货架稳定配方与厨房制备的 MDCF-2 相比均无显著差异。
MDCF-2 的包装、货架稳定原型在 8 至 12 个月大的孟加拉国儿童中表现出与新鲜制备的原始配方相当的可接受性。
