Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, PR China.
Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, PR China; Fruit and Vegetable Storage and Processing Technology Innovation Center of Shandong Province, Jinan Fruit Research Institute, All China Federation of Supply and Marketing Co-operatives, Jinan 250220, China.
Int J Biol Macromol. 2024 Oct;277(Pt 1):133696. doi: 10.1016/j.ijbiomac.2024.133696. Epub 2024 Jul 31.
For conventional emulsions used to encapsulate easily degradable bioactive compounds, achieving small droplet size and high encapsulation capacity is a challenging. Pickering emulsions stabilized by self-aggregated chitosan particles may offer high encapsulation efficiency due to the robust mechanical barrier formed by solid particles adsorbed at the oil-water interface. Therefore, the effects of pH, chitosan concentration, oil volume fraction, homogenization pressure, and homogenization cycle on the stability of chitosan Pickering emulsions and the degradation of β-carotene were investigated. Effective interfacial adsorption of chitosan nanoparticles and moderate homogenization intensity facilitated the formation of small emulsion droplets. Unlike conventional emulsions, chitosan Pickering emulsions with smaller droplets provided enhanced protection for β-carotene. This enhancement was primarily attributed to the improved interfacial coverage of chitosan nanoparticles with smaller droplet sizes, which was advantageous for β-carotene protection. The optimal conditions for preparing β-carotene-loaded chitosan Pickering emulsions were as follows: pH 6.5, chitosan concentration of 1.0 wt%, oil volume fraction of 20 %, homogenization pressure of 90 MPa, and 6 homogenization cycles. These findings indicate that chitosan Pickering emulsions are well-suited for encapsulating β-carotene with both small droplet size and high encapsulation efficiency.
对于用于封装易降解生物活性化合物的常规乳液，实现小的液滴尺寸和高的包封率是具有挑战性的。由自聚集壳聚糖颗粒稳定的 Pickering 乳液由于在油水界面吸附的固体颗粒形成了坚固的机械屏障，可能具有高的包封效率。因此，研究了 pH 值、壳聚糖浓度、油体积分数、均化压力和均化周期对壳聚糖 Pickering 乳液稳定性和 β-胡萝卜素降解的影响。壳聚糖纳米颗粒的有效界面吸附和适度的均化强度有利于形成小的乳液液滴。与常规乳液不同，具有较小液滴的壳聚糖 Pickering 乳液对 β-胡萝卜素提供了增强的保护。这种增强主要归因于较小液滴尺寸的壳聚糖纳米颗粒具有更好的界面覆盖，有利于 β-胡萝卜素的保护。制备负载 β-胡萝卜素的壳聚糖 Pickering 乳液的最佳条件为：pH 6.5、壳聚糖浓度 1.0wt%、油体积分数 20%、均化压力 90MPa 和 6 个均化循环。这些发现表明，壳聚糖 Pickering 乳液非常适合封装具有小液滴尺寸和高包封效率的 β-胡萝卜素。
