高效液相凝胶渗透色谱法后IgG的免疫荧光测定
Immunofluorescence determination of IgG following high performance liquid gel permeation chromatography.
作者信息
Hosotsubo H, Arai K, Iwamura J
出版信息
J Immunol Methods. 1985 Dec 17;85(1):115-9. doi: 10.1016/0022-1759(85)90279-0.
The application of high performance liquid gel permeation chromatography (HPL-GPC) and immunofluorescence for the estimation of a protein was studied using human IgG as a model system following separation of the fluorescent immune complex from free fluorescent-labelled antibody on a column of HPL-GPC. Using FITC-labelled anti-human IgG Fab fragment and HPL-GPC, nanogram levels of IgG could be determined within 5 min of mixing the antigen and antiserum. HPL-GPC immunofluorescence analysis is sensitive specific, simple, rapid and highly versatile.
以人免疫球蛋白G（IgG）作为模型系统，研究了高效液相凝胶渗透色谱法（HPL-GPC）和免疫荧光法在蛋白质定量分析中的应用。该方法是先在HPL-GPC柱上分离游离的荧光标记抗体与荧光免疫复合物。使用异硫氰酸荧光素（FITC）标记的抗人IgG Fab片段和HPL-GPC，在抗原与抗血清混合后5分钟内即可测定纳克水平的IgG。HPL-GPC免疫荧光分析灵敏、特异、简单、快速且通用性强。
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