Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan Province, Iran.
Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Antonie Van Leeuwenhoek. 2024 Aug 1;117(1):110. doi: 10.1007/s10482-024-02007-2.
Xenodidymella species have a wide range of hosts and can be found as pathogens and saprobes. In this study, two new species of Xenodidymella were found from leaf diseases of three pasture-medicinal plants in Ilam Province, in the west of Iran, and proposed here as X. ilamica and X. scandicis spp. nov. These species were identified based on morphological features and phylogenetic analyses of the internal transcribed spacer regions 1 & 2 and 5.8S nrDNA (ITS), partial beta-tubulin gene (tub2), and partial RNA polymerase II second largest subunit (rpb2) gene. The four Xenodidymella strains isolated in this study were delimited into two sister clades, with the two isolates of X. ilamica from the leaf spot of Colchicum speciosum and Ficaria kochii and two isolates of X. scandicis from leaf blight of Scandix pecten-veneris. Morphologically, X. scandicis produces larger, ostiolate or poroid pycnidia in vitro, while pycnidia in the cultures of X. ilamica are non-ostiolate and smaller. Some pycnidia in old cultures of X. scandicis produce a neck, but a distinct neck in X. ilamica has not been observed. Moreover, three plants under study are new hosts for the genus Xenodidymella.
多腔菌属物种宿主范围广泛，可作为病原菌和腐生物。本研究在伊朗西部伊拉姆省三种牧草植物的叶部病害中发现了两种新的多腔菌属物种，即伊拉姆多腔菌和沙氏多腔菌。这些物种是根据形态特征和 ITS（1&2 和 5.8S nrDNA）、β-微管蛋白基因（tub2）和 RNA 聚合酶 II 第二大亚基基因（rpb2）部分序列的系统发育分析确定的。本研究分离的四个多腔菌菌株被划分为两个姐妹分支，其中两个伊拉姆多腔菌分离株来自 Colchicum speciosum 和 Ficaria kochii 的叶斑病，两个沙氏多腔菌分离株来自 Scandix pecten-veneris 的叶枯病。形态上，沙氏多腔菌在体外产生较大的、具孔口或多孔的分生孢子器，而伊拉姆多腔菌的分生孢子器无孔口且较小。沙氏多腔菌的一些老培养物中的分生孢子器产生颈，但在伊拉姆多腔菌中没有观察到明显的颈。此外，研究中的三种植物是多腔菌属的新宿主。
