University of Debrecen.
Institute of Agricultural Chemistry and Soil Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
Int J Med Mushrooms. 2024;26(9):77-87. doi: 10.1615/IntJMedMushrooms.2024054590.
This study aimed to examine the impact of mushroom extract-based solid media on the β-glucan content, growth rate, density, and biomass content of Pleurotus ostreatus (oyster mushroom) mycelia. Fresh, high-quality raw P. ostreatus were washed, sliced, and heated in a sealed pressure cooker at 90°C for 4 h in the drying cabinet. Following the heating process, centrifugation was carried out. Different concentrations of Pleurotus ostreatus extract were mixed with distilled water (0%, 25%, 50%, 75%, and 100%) and prepared for a sterile solid media. A malt extract-based medium was maintained as a control. This study focuses on the growth performance of P. ostreatus mycelium on its own mushroom extract-based culture medium which holds considerable economic and environmental significance. During the six-day observation period, the mycelium exhibited consistent growth across all tested media, maintaining a steady growth rate of 15 mm. The increased content of mushroom extract resulted from the enhanced density of the mycelia and biomass content. It can be inferred that when media containing less than 25% of mushroom extract dilution is used, β-glucan can be formed in smaller amounts. Further research is needed to explore mushroom extract derived from different types of mushroom byproducts, which fail to meet commercial standards.
本研究旨在考察基于蘑菇提取物的固体培养基对糙皮侧耳（平菇）菌丝体β-葡聚糖含量、生长速度、密度和生物量的影响。新鲜、高质量的糙皮侧耳经清洗、切片后，在干燥箱中于 90°C 下用密封高压锅加热 4 小时。加热后进行离心。不同浓度的糙皮侧耳提取物与蒸馏水（0%、25%、50%、75%和 100%）混合，用于无菌固体培养基的制备。以麦芽提取物为基础的培养基作为对照。本研究侧重于糙皮侧耳菌丝体在其自身基于蘑菇提取物的培养基上的生长性能，这具有相当大的经济和环境意义。在六天的观察期内，所有测试的培养基中菌丝体都表现出一致的生长，保持稳定的 15mm 生长速度。由于菌丝体密度和生物量的增加，蘑菇提取物的含量增加。可以推断，当使用含有低于 25%蘑菇提取物稀释液的培养基时，β-葡聚糖的形成量会减少。需要进一步研究探索来自不同类型蘑菇副产物的蘑菇提取物，这些提取物不符合商业标准。
