Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City and Southwest University, Chongqing Key Laboratory of Crop Quality Improvement, College of Agronomy and Biotechnology, Southwest University, Chongqing, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Science, Southwest University, Chongqing, China.
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City and Southwest University, Chongqing Key Laboratory of Crop Quality Improvement, College of Agronomy and Biotechnology, Southwest University, Chongqing, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Science, Southwest University, Chongqing, China.
Ecotoxicol Environ Saf. 2024 Sep 15;283:116792. doi: 10.1016/j.ecoenv.2024.116792. Epub 2024 Aug 3.
Cytochromes P450 monooxygenases (CYP450s) constitute the largest enzymic protein family that is widely present in plants, animals, and microorganisms, participate in numerous metabolic pathways, and play diverse roles in development, metabolism, and defense. Rapeseed (Brassica napus) is an important oil crop worldwide and have many versions of reference genome. However, there is no systemically comparative genome-wide analysis of CYP450 family genes in rapeseed and its parental species B. rapa and B. oleracea. In this study, we identified 765, 293 and 437 CYP450 genes in B. napus, B. rapa and B. oleracea, respectively, which were unevenly located in A01-A10 and/or C01-C09 chromosomes in corresponding species. Phylogenetic relationship analysis indicated that 1745 CYP450 proteins from three Brassica species and Arabidopsis were divided into 4 groups. Whole genome duplication (WGD) or segmental duplication resulted in gene expansion of CYP450 family in three Brassica species. There were 33-83 SSR loci in CYP450 genes of three Brassica species, and numerous transcription factor binding sites were identified in their promoters. A total of 459-777 miRNAs were predicted to target 174-426 CYP450 genes in three Brassica species. Based on transcriptome data, BnCYP450s, BrCYP450s and BoCYP450s were differentially expressed in various tissues. There existed numerous BnCYP450 DEGs in response to pathogens and abiotic stresses. Besides, many BnCYP450 DEGs were involved in the regulation of important traits, such as seed germination, seed ALA content, and yellow-seed. The qRT-PCR experiment confirmed the transcriptome analysis results by validating two representative Sclerotinia-responsive BnCYP450 DEGs as an example. Three BnCYP450s genes (CYP707A1, CYP81F1, CYP81H1) might be regulated by seed-specific transcription factors BnTT1 and BnbZIP67 to participate in the development and metabolism of seed coat and embryo by undertaking related metabolic reactions.
细胞色素 P450 单加氧酶 (CYP450s) 构成了最大的酶蛋白家族，广泛存在于植物、动物和微生物中，参与众多代谢途径，并在发育、代谢和防御中发挥多种作用。油菜 (Brassica napus) 是世界上一种重要的油料作物，拥有许多参考基因组版本。然而，油菜及其亲本甘蓝型油菜 (B. rapa) 和白菜型油菜 (B. oleracea) 中的 CYP450 家族基因尚未进行系统的全基因组比较分析。在本研究中，我们分别在油菜、甘蓝型油菜和白菜型油菜中鉴定到 765、293 和 437 个 CYP450 基因，这些基因不均匀地定位在相应物种的 A01-A10 和/或 C01-C09 染色体上。系统发育关系分析表明，来自 3 种芸薹属植物和拟南芥的 1745 个 CYP450 蛋白被分为 4 组。全基因组复制 (WGD) 或片段复制导致 3 种芸薹属植物 CYP450 家族基因的扩张。3 种芸薹属植物 CYP450 基因中存在 33-83 个 SSR 位点，并且在其启动子中鉴定到大量转录因子结合位点。共预测到 459-777 个 miRNA 靶向 3 种芸薹属植物的 174-426 个 CYP450 基因。基于转录组数据，BnCYP450s、BrCYP450s 和 BoCYP450s 在不同组织中呈现差异表达。存在大量 BnCYP450 DEGs 响应于病原体和非生物胁迫。此外，许多 BnCYP450 DEGs 参与了重要性状的调控，如种子萌发、种子 ALA 含量和黄籽。qRT-PCR 实验通过验证两个代表性的 Sclerotinia 响应的 BnCYP450 DEGs 作为一个例子，证实了转录组分析结果。三个 BnCYP450s 基因 (CYP707A1、CYP81F1、CYP81H1) 可能受到种子特异性转录因子 BnTT1 和 BnbZIP67 的调控，通过承担相关的代谢反应，参与种皮和胚的发育和代谢。
