两种氨基转移酶和D-氨基酸氧化酶在幼年和成年大鼠肾单位内的分布。

Distribution of two aminotransferases and D-amino acid oxidase within the nephron of young and adult rats.

作者信息

Chan A W, Perry S G, Burch H B, Fagioli S, Alvey T R, Lowry O H

出版信息

J Histochem Cytochem. 1979 Mar;27(3):751-5. doi: 10.1177/27.3.39098.

DOI:10.1177/27.3.39098

Abstract

In the adult rat kidney, alanine aminotransferase (EC 2.6.1.2), aspartate aminotransferase (EC 2.6.1.1) and D-amino acid oxidase (EC 1.4.3.3) were measured in glomeruli, 4 parts of the proximal tubule, 2 parts of the distal tubule and in patches from the thin limb area and the papilla. These enzymes were measured in more limited parts of the nephron during postnatal development. Adult aspartate aminotransferase activities (percentage of the highest) ranged from 100 in the distal straight segment to 25 in the late part of the proximal straight segment to 10 in the thin limb and papillary area. Alanine aminotransferase (lower by a factor of 100 in absolute terms) was distributed as the mirror image of aspartate aminotransferase within proximal and distal tubules. D-Amino acid oxidase was 850-fold higher in proximal straight segments than in medullary structures. During development alanine aminotransferase increased 6-fold and D-amino acid oxidase, 4.5-fold in proximal straight tubules but aspartate aminotransferase increased in distal straight tubles 8-fold.

摘要

在成年大鼠肾脏中，测定了肾小球、近端小管的4个部分、远端小管的2个部分以及来自细段区域和乳头的小块组织中的丙氨酸转氨酶（EC 2.6.1.2）、天冬氨酸转氨酶（EC 2.6.1.1）和D-氨基酸氧化酶（EC 1.4.3.3）。在出生后发育过程中，在肾单位更有限的部分测定了这些酶。成年天冬氨酸转氨酶活性（最高值的百分比）范围从远端直段的100%到近端直段后期的25%，再到细段和乳头区域的10%。丙氨酸转氨酶（绝对值低100倍）在近端和远端小管内的分布与天冬氨酸转氨酶呈镜像关系。近端直段的D-氨基酸氧化酶比髓质结构中的高850倍。在发育过程中，近端直小管中的丙氨酸转氨酶增加了6倍，D-氨基酸氧化酶增加了4.5倍，但远端直小管中的天冬氨酸转氨酶增加了8倍。