通过缺口 DNA 衔接物介导的连接实现有效的环状 RNA 合成。

Efficient circular RNA synthesis through Gap-DNA splint-mediated ligation.

机构信息

Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of Korea.

出版信息

Nanoscale. 2024 Aug 22;16(33):15529-15532. doi: 10.1039/d4nr01770f.

DOI:10.1039/d4nr01770f

PMID:39102212

Abstract

The COVID-19 pandemic heightened interest in circular RNA (C-RNA) for RNA therapeutics, offering advantages over linear mRNAs. Circular mRNA facilitates uncapped molecule development, and C-RNAs ensure stability in RNA interference therapeutics. The synthesis method, RNA ligation, is employed in C-RNA-based therapeutics. Stable DNA-RNA hybrid constructs enable efficient RNA ligase-based circularization.

摘要

新冠疫情提高了人们对环状 RNA（C-RNA）在 RNA 治疗中的兴趣，它比线性 mRNA 具有优势。环状 mRNA 促进无帽分子的发展，而 C-RNAs 确保 RNA 干扰治疗的稳定性。基于 C-RNA 的治疗方法采用了 RNA 连接的合成方法。稳定的 DNA-RNA 杂交构建体可实现基于高效 RNA 连接酶的环状化。