Department of Ultrasound, Second Affiliated Hospital of Fujian Medical University, Zhongshan Road, Licheng District, Quanzhou, 362000, China.
Department of Ultrasound, Quanzhou Hospital of Traditional Chinese Medicine, Quanzhou, 362000, China.
Sci Rep. 2024 Aug 5;14(1):18133. doi: 10.1038/s41598-024-69051-w.
To study a new method for establishing animal models of prenatal bronchopulmonary dysplasia (BPD), we used lung ultrasound score (LUS) to semi-quantitatively assess the severity of lung lesions in model rats. Lipopolysaccharide (LPS) was injected into the right lung of the fetus of the rat under ultrasound-guided, and the right lung of the neonates were scanning for LUS. Specimens were collected for pathological scoring and detection of pulmonary surfactant-associated glycoprotein (SP)-C and vascular endothelial growth factor (VEGF) expression quantity. The correlation between LUS and pathological scores was analyzed. (1) The animal models were consistent with the pathological manifestations of BPD. (2) It showed a strong positive correlation between LUS and pathological scores in animal models (r = 0.84, P < 0.005), and the expression quantity of SP-C and VEGF in lung tissue were decreased (both P < 0.05). Animal models established by ultrasound-guided puncture of the lung of rats and injection of LPS were consistent with the manifestation of BPD. This method could be used to establish animal models of BPD before birth, and the severity of BPD could be assessed by using LUS.
为了研究一种新的建立产前支气管肺发育不良（BPD）动物模型的方法，我们使用肺部超声评分（LUS）来半定量评估模型大鼠肺部病变的严重程度。在超声引导下向胎鼠的右肺内注射脂多糖（LPS），对新生鼠的右肺进行 LUS 扫描。收集标本进行病理评分，并检测肺表面活性物质相关蛋白 C（SP-C）和血管内皮生长因子（VEGF）的表达量。分析 LUS 与病理评分之间的相关性。（1）动物模型与 BPD 的病理表现一致。（2）动物模型中 LUS 与病理评分之间呈强正相关（r=0.84，P<0.005），肺组织中 SP-C 和 VEGF 的表达量降低（均 P<0.05）。超声引导下穿刺胎鼠肺并注射 LPS 建立的动物模型与 BPD 的表现一致。该方法可用于建立产前 BPD 动物模型，并可通过 LUS 评估 BPD 的严重程度。
