Yousef A Alnakeeb Ebtisam, Abdull Razis Ahmad Faizal, Wei Chan Kim, Ling Tham Chau, Han Chan Yee, Kalifa Kafo Anwar Salm, Jambari Nuzul Noorahya, Rollin Patrick, Djedaini-Pilard Florence
Natural Medicines and Products Research Laboratory Institute of Bioscience Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia.
Laboratory of Food Safety and Food Integrity Institute of Tropical Agriculture and Food Security Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia.
Adv Pharmacol Pharm Sci. 2024 Jul 25;2024:8885068. doi: 10.1155/2024/8885068. eCollection 2024.
Allergic diseases (ADs) are a major concern when it comes to public well-being. Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from seeds in the form of alpha-cyclodextrin-moringin (-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The -CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and -CD/MG. The release of beta-hexosaminidase (-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-), and interleukin (IL-4) was considered in the late stage. Higher concentrations of -CD/MG (5 M, < 0.001) in mast cell degranulation significantly inhibited the expression of -hexosaminidase, histamine, TNF-, PGD2, and IL-4 in both the early and late stages. Thus, -CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.
过敏性疾病是关乎公众健康的重大问题。辣木是一种热带植物，因其含有异硫氰酸酯而被用于传统医学。本研究调查了从种子中分离出的4-(-L-鼠李糖基氧基)-苄基异硫氰酸酯或辣木素以α-环糊精-辣木素（-CD/MG）复合物形式对大鼠嗜碱性白血病（RBL-2H3）细胞系在过敏反应早期和晚期的抗过敏特性。-CD/MG复合物最初通过核磁共振（NMR）进行阐明，随后采用3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑内盐增殖试验来评估相对于富马酸酮替芬（KF）和-CD/MG的细胞毒性和细胞活力。β-己糖胺酶（-己糖胺酶）和组胺的释放用于确定早期的抑制水平，而晚期则考虑抑制前列腺素（PGD2）、肿瘤坏死因子-α（TNF-）和白细胞介素（IL-4）的释放。较高浓度的-CD/MG（5μM，P<0.001）在肥大细胞脱颗粒过程中显著抑制了早期和晚期的-己糖胺酶、组胺、TNF-、PGD2和IL-4的表达。因此，-CD/MG具有在早期和晚期抑制过敏反应的能力，有可能被开发为一种抗过敏药物。
