Rammali Said, Ciobică Alin, El Aalaoui Mohamed, Rahim Abdellatif, Kamal Fatima Zahra, Dari Khadija, Khattabi Abdelkrim, Romila Laura, Novac Bogdan, Petroaie Antoneta, Bencharki Bouchaib
Laboratory of Agro-Alimentary and Health, Faculty of Sciences and Techniques, Hassan First University of Settat, Settat, Morocco.
Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, Iași, Romania.
Front Microbiol. 2024 Jul 22;15:1429035. doi: 10.3389/fmicb.2024.1429035. eCollection 2024.
The alarming rise in antimicrobial resistance (AMR) has created a significant public health challenge, necessitating the discovery of new therapeutic agents to combat infectious diseases and oxidative stress-related disorders. The strain E25-2, isolated from Moroccan forest soil, represents a potential avenue for such research. This study aimed to identify the isolate E25-2, obtained from soil in a cold Moroccan ecosystem, and further investigate its antimicrobial and antioxidant activities. Phylogenetic analysis based on 16S rRNA gene sequences revealed the strain's classification within the genus, with a sequence closely resembling that of AS4.0578 (96.10% similarity). Antimicrobial activity in solid media showed moderate to strong activity against ATCC 25923, strain ATCC 14579, strain ATCC 25922, strain ATCC 60193 and 4 phytopathogenic fungi. In addition, ethyl acetate extract of this isolate demonstrated potent antimicrobial activity against 7 clinically multi-drug resistant bacteria. Furthermore, it demonstrated antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals, as well as a significant increase in ferric reducing antioxidant power. A significant positive correlation was observed between antioxidant activities and total content of phenolic compounds ( < 0.0001), along with flavonoids ( < 0.0001). Furthermore, gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of amines, hydroxyl groups, pyridopyrazinone rings, esters and pyrrolopyrazines. The genus could offer promising prospects in the fight against antibiotic resistance and in the prevention against oxidative stress related diseases.
抗菌药物耐药性（AMR）的惊人上升带来了重大的公共卫生挑战，因此需要发现新的治疗药物来对抗传染病和与氧化应激相关的疾病。从摩洛哥森林土壤中分离出的E25-2菌株是此类研究的一个潜在途径。本研究旨在鉴定从摩洛哥寒冷生态系统土壤中获得的分离株E25-2，并进一步研究其抗菌和抗氧化活性。基于16S rRNA基因序列的系统发育分析揭示了该菌株在该属内的分类，其序列与AS4.0578的序列密切相似（相似度为96.10%）。在固体培养基中的抗菌活性显示对ATCC 25923、菌株ATCC 14579、菌株ATCC 25922、菌株ATCC 60193和4种植物病原真菌具有中度至强活性。此外，该分离株的乙酸乙酯提取物对7种临床多重耐药细菌表现出强大的抗菌活性。此外，它还对2,2-二苯基-1-苦基肼（DPPH）和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基表现出抗氧化活性，以及铁还原抗氧化能力的显著增加。抗氧化活性与酚类化合物（<0.0001）以及黄酮类化合物（<0.0001）的总含量之间存在显著正相关。此外，气相色谱-质谱（GC-MS）分析揭示了胺、羟基、吡啶并吡嗪酮环、酯和吡咯并吡嗪的存在。该属在对抗抗生素耐药性和预防与氧化应激相关疾病方面可能具有广阔前景。
