State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Front Cell Infect Microbiol. 2024 Jul 22;14:1422560. doi: 10.3389/fcimb.2024.1422560. eCollection 2024.
Porcine epidemic diarrhea virus (PEDV) is associated with severe enteritis, which contributes to high mortality in piglets. The aim of this study was to describe molecular mechanisms associated with proinflammatory cytokine(s) production during PEDV infection. We showed that infection of porcine intestine epithelial cell clone J2 (IPEC-J2) with PEDV induces a gradual increase in interleukin 8 (IL-8) production at different time points, as well as infection of Vero E6 with PEDV. The secretion of IL-8 in these two cell lines infected with PEDV is related to the activation of NF-κB. Furthermore, the cells expressing PEDV M or E protein can induce the upregulation of IL-8. These findings suggest that the IL-8 production can be the initiator of inflammatory response by the host cells upon PEDV infection.
猪流行性腹泻病毒（PEDV）与严重的肠炎有关，这导致仔猪死亡率很高。本研究旨在描述 PEDV 感染过程中与促炎细胞因子产生相关的分子机制。我们表明，猪肠上皮细胞克隆 J2（IPEC-J2）感染 PEDV 会在不同时间点逐渐增加白细胞介素 8（IL-8）的产生，同时 Vero E6 感染 PEDV 也是如此。这两种细胞系感染 PEDV 后 IL-8 的分泌与 NF-κB 的激活有关。此外，表达 PEDV M 或 E 蛋白的细胞可以诱导 IL-8 的上调。这些发现表明，IL-8 的产生可能是宿主细胞感染 PEDV 后炎症反应的启动者。
