Abdelaziz Tarek A, Mesbah Noha M, Abo-Elmatty Dina M, El-Sabbagh Farah O
Department of Cardiology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Arch Physiol Biochem. 2025 Feb;131(1):33-39. doi: 10.1080/13813455.2024.2387691. Epub 2024 Aug 6.
Coronary artery spasm is among the etiology of myocardial infarction. Oxidative stress is involved in the pathogenesis of coronary artery spasm (CAS). Paraoxonase-1 (PON1) is an HDL-bound antioxidant enzyme that protects LDL from oxidative modification. Oxidative-stress-related genetic factors and certain polymorphisms in the paraoxonase 1 gene might influence the pathogenesis of CAS. We aimed to investigate the association between PON1 gene polymorphism and its enzymatic activity and coronary artery spasm during cardiac catheterization.
The study population was 150 patients who underwent elective coronary angiography. Subjects were genotyped to the Q192R polymorphism (rs662) on the PON1 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and PON1 activity was quantitatively analyzed by enzyme linked immunosorbent assay. Results showed that the subjects carrying the RR genotype and R allele were significantly more likely to develop coronary artery spasm (OR=4.2, 2.03, < 0.006, ˂0.02, respectively). Moreover, serum PON1 levels were significantly decreased (˂0.001) in the CAS group. RR genotype of PON1 Q192R polymorphism, Tc, LDLc, TG, catheter size, and paroxonase-1 serum level are independent predictors of coronary spasm.
We conclude that the PON1 (rs662) gene polymorphism is associated with CAS during cardiac catheterization in Egyptians. The PON1-192R allele and lower serum enzyme concentration may play an important role in coronary spasm.
冠状动脉痉挛是心肌梗死的病因之一。氧化应激参与冠状动脉痉挛（CAS）的发病机制。对氧磷酶-1（PON1）是一种与高密度脂蛋白（HDL）结合的抗氧化酶，可保护低密度脂蛋白（LDL）免受氧化修饰。氧化应激相关的遗传因素和对氧磷酶1基因中的某些多态性可能影响CAS的发病机制。我们旨在研究对氧磷酶1基因多态性及其酶活性与心脏导管插入术中冠状动脉痉挛之间的关联。
研究人群为150例行择期冠状动脉造影的患者。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对PON1基因的Q192R多态性（rs662）进行基因分型，并通过酶联免疫吸附测定法定量分析PON1活性。结果显示，携带RR基因型和R等位基因的受试者发生冠状动脉痉挛的可能性显著更高（OR分别为4.2和2.03，P均<0.006和˂0.02）。此外，CAS组的血清PON1水平显著降低（˂0.001）。PON1 Q192R多态性的RR基因型、总胆固醇（Tc）、低密度脂蛋白胆固醇（LDLc）、甘油三酯（TG）、导管尺寸和对氧磷酶-1血清水平是冠状动脉痉挛的独立预测因素。
我们得出结论，在埃及人中，PON1（rs662）基因多态性与心脏导管插入术中的CAS相关。PON1 - 192R等位基因和较低的血清酶浓度可能在冠状动脉痉挛中起重要作用。
