Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.
Department of Animal Nutrition, Faculty of Veterinary Medicine, Bingol University, Bingol, Turkey.
Clin Exp Med. 2024 Aug 6;24(1):176. doi: 10.1007/s10238-024-01434-9.
Biotin and arginine play crucial roles in lipid metabolism and may offer promising interventions against obesity. This study examined the combined effect of magnesium biotinate (MgB) and inositol-stabilized arginine silicate complex (ASI) on obesity-related oxidative imbalance, inflammation, lipid metabolism and neuromodulation in rats on a high-fat diet (HFD). Forty rats were divided into five groups: (a) control: rats were fed a standard diet containing 12% of energy from fat; (b) HFD: rats were fed the HFD with 42% of energy from fat; (c) HFD + MgB: rats were fed the HFD and given 0.31 mg/kg body weight (BW) MgB, (d) HFD + ASI: rats were fed the HFD and were given 12.91 mg/kg BW ASI), and (e) HFD + MgB + ASI: rats were fed the HFD and given 0.31 mg/kg BW MgB and 12.91 mg/kg BW ASI). The combined administration of MgB and ASI reduced the levels of serum cholesterol, free fatty acid (FFA), and malondialdehyde (MDA), as well as liver inflammatory cytokines, sterol regulatory element-binding protein 1-c (SREBP-1c), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) proteins (P < 0.001) compared to HFD rats without supplementation. Moreover, this combination increased the activities of antioxidant enzymes (P < 0.05) and boosted the brain-derived neurotrophic factor (BDNF), serotonin, dopamine (P < 0.001), as well as liver insulin receptor substrate 1 (IRS-1) and peroxisome proliferator-activated receptor gamma (PPAR-γ) (P < 0.001). These findings suggest that combining MgB and ASI could deter liver fat accumulation and enhance lipid metabolism in HFD-fed rats by modulating various metabolic pathways and neuromodulators related to energy metabolism. This combination demonstrates potential in addressing obesity and its related metabolic dysfunctions.
生物素和精氨酸在脂质代谢中发挥着关键作用，可能为肥胖症的防治提供有前景的干预措施。本研究探讨了生物素镁（MgB）和肌醇稳定的硅酸精氨酸复合物（ASI）联合应用对高脂肪饮食（HFD）大鼠肥胖相关氧化失衡、炎症、脂质代谢和神经调节的影响。将 40 只大鼠分为 5 组：（a）对照组：大鼠喂食含 12%脂肪能量的标准饮食；（b）HFD 组：大鼠喂食含 42%脂肪能量的 HFD；（c）HFD+MgB 组：大鼠喂食 HFD 并给予 0.31mg/kg 体重（BW）MgB；（d）HFD+ASI 组：大鼠喂食 HFD 并给予 12.91mg/kg BW ASI）；（e）HFD+MgB+ASI 组：大鼠喂食 HFD 并给予 0.31mg/kg BW MgB 和 12.91mg/kg BW ASI）。与未补充的 HFD 大鼠相比，MgB 和 ASI 的联合给药降低了血清胆固醇、游离脂肪酸（FFA）和丙二醛（MDA）水平，以及肝炎性细胞因子、固醇调节元件结合蛋白 1-c（SREBP-1c）和 3-羟-3-甲基戊二酰辅酶 A 还原酶（HMGR）蛋白（P<0.001）。此外，这种组合增加了抗氧化酶的活性（P<0.05），并促进了脑源性神经营养因子（BDNF）、血清素、多巴胺（P<0.001），以及肝胰岛素受体底物 1（IRS-1）和过氧化物酶体增殖物激活受体γ（PPAR-γ）（P<0.001）。这些发现表明，MgB 和 ASI 的联合应用可以通过调节与能量代谢相关的各种代谢途径和神经调节剂来阻止 HFD 喂养大鼠的肝脂肪积累并增强脂质代谢。这种组合在解决肥胖及其相关代谢功能障碍方面具有潜力。
