Department of Otorhinolaryngology head and neck surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Department of Otorhinolaryngology head and neck surgery, The Second Affiliated Hospital of Xi 'an Medical University, Xi'an, 710000, Shaanxi, China.
BMC Biotechnol. 2024 Aug 6;24(1):53. doi: 10.1186/s12896-024-00877-y.
Chemotherapy as a cornerstone of cancer treatment is slowly being edged aside owing to its severe side effects and systemic toxicity. In this case, nanomedicine has emerged as an effective tool to address these drawbacks. Herein, a biocompatible carrier based on bovine serum albumin (BSA) coated gadolinium oxide nanoparticles (GdO@BSA) was fabricated for curcumin (CUR) delivery and its physicochemical features along with its potential anticancer activity against nasal squamous cell carcinoma were also investigated. It was found that the fabricated GdO@BSA containing CUR (GdO@BSA-CUR) had spherical morphology with hydrodynamic size of nearly 26 nm, zeta-potential of -36 mV and high drug (CUR) loading capacity. Drug release profile disclosed that the release of CUR from the prepared GdO@BSA-CUR nanoparticles occurred in a sustained- and pH-dependent manner. Also, in vitro cytotoxicity analysis revealed that the fabricated GdO@BSA nanoparticles possessed excellent biosafety toward HFF2 normal cells, while GdO@BSA-CUR appeared to display the greatest anticancer potential against RPMI 2650 and CNE-1 cancer cell lines. The results also show that the GdO@BSA nanoparticles were compatible with the blood cells with minor hemolytic effect (< 3%). The manufactured NPs were found to be completely safe for biological applications in an in vivo subacute toxicity study. Taken together, these finding substantiate the potential anticancer activity of GdO@BSA-CUR nanoparticles against nasal squamous cell carcinoma, but the results obtained demand further studies to assess their full potential.
化疗作为癌症治疗的基石，由于其严重的副作用和全身毒性，正逐渐被边缘化。在这种情况下，纳米医学已成为解决这些问题的有效工具。本文制备了一种基于牛血清白蛋白（BSA）包覆的氧化钆纳米粒子（GdO@BSA）的生物相容性载体，用于姜黄素（CUR）的递送，并对其理化特性及其对鼻腔鳞状细胞癌的潜在抗癌活性进行了研究。结果发现，制备的载有 CUR 的 GdO@BSA（GdO@BSA-CUR）具有近 26nm 的球形形态、-36mV 的动电势和高药物（CUR）负载能力。药物释放曲线表明，制备的 GdO@BSA-CUR 纳米粒子中 CUR 的释放呈持续且 pH 依赖性释放。此外，体外细胞毒性分析表明，制备的 GdO@BSA 纳米粒子对 HFF2 正常细胞具有优异的生物安全性，而 GdO@BSA-CUR 对 RPMI 2650 和 CNE-1 癌细胞系显示出最大的抗癌潜力。结果还表明，GdO@BSA 纳米粒子与血细胞相容，溶血作用较小（<3%）。在体内亚急性毒性研究中，发现所制备的 NPs 完全适用于生物应用。综上所述，这些发现证实了 GdO@BSA-CUR 纳米粒子对鼻腔鳞状细胞癌的潜在抗癌活性，但获得的结果需要进一步研究以评估其全部潜力。
