临床分离的白喉棒状杆菌菌株产生卟啉的情况。

Porphyrin Production by Corynebacterium diphtheriae Strains from Clinical Isolates.

作者信息

Alves Gabriela Batista, da Costa Marques Calderari Mônica Regina, da Fonseca Eduardo Nunes, Dos Santos Louisy Sanches, de Mattos-Guaraldi Ana Luiza

机构信息

Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Rio de Janeiro State University, Av. 28 de Setembro, 87 - Fundos, 3° andar, Vila Isabel, RJ, CEP 20 551-030, Brazil.

General and Inorganic Chemistry Laboratory, Federal Institute of Education, Science and Technology of Rio de Janeiro, Rua Senador Furtado, 121, Maracanã, Rio de Janeiro, RJ, CEP 20270-021, Brazil.

出版信息

Chem Biodivers. 2024 Dec;21(12):e202401011. doi: 10.1002/cbdv.202401011. Epub 2024 Oct 18.

DOI:10.1002/cbdv.202401011

PMID:39110090

Abstract

Porphyrins are intermediate metabolites involved in the biosynthesis of vital molecules, including heme, cobalamin, and chlorophyll. Bacterial porphyrins are known to be proinflammatory and are associated with biofilm formation. This study investigated porphyrin production by strains of Corynebacterium diphtheriae using emission spectroscopy, high-performance liquid chromatography with fluorescence detection, diode array detector, and mass spectrometry. Emission spectroscopy revealed characteristic porphyrin emission spectra in all strains, with coproporphyrin III predominating. Qualitative analysis by different chromatographic methods identified coproporphyrin III, uroporphyrin I, and protoporphyrin IX in all strains. Quantitative analysis revealed strain-dependent coproporphyrin III production. Further studies are required to investigate the relationship between porphyrin production and the virulence potential of Corynebacterium diphtheriae.

摘要

卟啉是参与重要分子生物合成的中间代谢产物，包括血红素、钴胺素和叶绿素。已知细菌卟啉具有促炎作用，并与生物膜形成有关。本研究使用发射光谱法、带荧光检测的高效液相色谱法、二极管阵列检测器和质谱法，对白喉棒状杆菌菌株的卟啉生成情况进行了调查。发射光谱显示所有菌株中均有特征性的卟啉发射光谱，其中粪卟啉原III占主导。通过不同色谱方法进行的定性分析在所有菌株中均鉴定出了粪卟啉原III、尿卟啉原I和原卟啉IX。定量分析显示粪卟啉原III的生成具有菌株依赖性。需要进一步研究来探讨卟啉生成与白喉棒状杆菌毒力潜能之间的关系。