Evolutionary theory predicts that the species of an evolutionarily successful taxon would not overlap in spatial distribution. To test the prediction, we document our research on the spatial associations of mustelids, an evolutionarily successful group of order Carnivore, using infrared camera trap data on species distribution collected from the national nature reserves (NNRs) of Liancheng, Wolong, Tangjiahe and Heizhugou in China in 2017-2021. Data showed seven mustelid species occurring in the study area, including , , , , , and . Following Ricklef's definition of biological community, we identified five networks of species associations. The mustelids occurred in the networks. Species from the same genus, such as and , stayed in different networks to avoid competition owing to similar feeding habits or habitat preferences. Species with different feeding habits or habitat preferences either occurred in different networks, such as and , or coexisted in the same networks but avoided direct spatial associations, such as and . Asymmetrical associations were found between different genera, such as and , or between different subfamilies, such as and . These associations may be attributed to interspecific killing or seed dispersal. However, these associations accounted for only a small proportion and would not impact the species diversity of Mustelidae. It is thus concluded that the prediction is supported by our research findings and that spatial avoidance may be the biogeographic strategy of maintaining the species diversity of the family. We also found that the well protection of the mustelids may benefit the overall biodiversity conservation in Heizhugou, an NNR that has experienced severe deforestation.