Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, India.
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai, India.
Microsc Res Tech. 2024 Dec;87(12):3003-3015. doi: 10.1002/jemt.24653. Epub 2024 Aug 8.
The present study unveils the intricate details on the morphology of thrips through optical, field emission scanning electron microscopy (FE-SEM) and mitochondrial cytochrome oxidase I gene-based molecular identification tools. The variation in the morphological characters namely, antennae (seven-segmented with forked sensorium on third, fourth segments), ctenidia (paired ctenidia were present in 5-8 abdominal segments laterally), pronotum (two pairs of posteroangular setae) were observed in both Thrips tabaci and Thrips parvispinus, respectively. Similarly, ocelli color (brown and red colored), ocellar setae (two and three pairs of ocellar setae on the head of T. tabaci and T. parvispinus, respectively. Irregular reticulate striations on metascutum and medial striations are present in the metanotum of T. parvispinus; forewings with 6 distal setae in the first vein and 15 distal setae in the second vein in T. tabaci and forewings of T. parvispinus with complete rows of setae in the first and second vein in T. parvispinus; abdomen with median dorsal setae present in the tergite of T. tabaci and presence of 6-12 discal setae in sternites III-VI segments, absence of discal setae on sternites II and VII in T. parvispinus were observed, respectively. Further, FE-SEM studies revealed that similar type of sensilla namely, sensilla basiconica (SBI, SBII, SBIII), sensilla chaetica (SChI, SChII), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav) were recorded in both the species and variations were observed in length of above sensilla of T. tabaci and T. parvispinus. Additionally, Bohm bristles (Bb) and microtrichia (Mt) on the antennal surface contributed to a comprehensive understanding of their ultrastructural features. The molecular characterization revealed a single ~450 bp nucleotide fragment with over 98% similarity for the confirmation of T. tabaci and T. parvispinus in concurrence with NCBI data. RESEARCH HIGHLIGHTS: Microscopy-based morphological and ultrastructural characterization of Thrips tabaci Lindeman and Thrips parvispinus Karny.
本研究通过光学显微镜、场发射扫描电子显微镜（FE-SEM）和基于线粒体细胞色素氧化酶 I 基因的分子鉴定工具揭示了蓟马形态的详细信息。在触角（第三、四段有分叉感器的七节）、纤毛（5-8 个腹部段侧面有成对的纤毛）、前胸（两对后角刚毛）等形态特征上，烟粉虱和短管蓟马分别存在差异。同样，眼点颜色（棕色和红色）、眼点刚毛（烟粉虱和短管蓟马头部的两对和三对眼点刚毛）、中胸盾片上不规则的网状条纹和中胸盾片上的中线条纹在短管蓟马中存在；烟粉虱前翅第一静脉有 6 根远端刚毛，第二静脉有 15 根远端刚毛，而短管蓟马前翅第一和第二静脉都有完整的刚毛排列；烟粉虱腹部的中背刚毛存在于第 10 背板，第 3-6 腹板有 6-12 根节间背侧刚毛，而短管蓟马的第 2 和第 7 腹板没有节间背侧刚毛，第 3-6 腹板有 6-12 根节间背侧刚毛。此外，FE-SEM 研究表明，两种蓟马都存在类似的感觉器，即锥形感觉器（SBI、SBII、SBIII）、毛状感觉器（SChI、SChII）、刚毛感觉器（ST）、钟形感觉器（SCa）和腔感觉器（SCav），并且烟粉虱和短管蓟马的上述感觉器长度存在差异。此外，触角表面的博姆刚毛（Bb）和微纤毛（Mt）有助于全面了解它们的超微结构特征。分子特征分析证实了烟粉虱和短管蓟马的存在，得到了一个约 450bp 核苷酸片段，与 NCBI 数据的相似度超过 98%。研究亮点：烟粉虱和短管蓟马的基于显微镜的形态学和超微结构特征。
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