基于安培生物传感器检测炭疽芽孢的研究进展
Development of an amperometric biosensor for the detection of Bacillus anthracis spores.
机构信息
Department of Medical CBRN Defense, University of Health Sciences, 06018, Ankara Türkiye.
Department of Biology, Faculty of Science, Erciyes University, 38039, Kayseri, Türkiye.
出版信息
Diagn Microbiol Infect Dis. 2024 Nov;110(3):116473. doi: 10.1016/j.diagmicrobio.2024.116473. Epub 2024 Aug 7.
INTRODUCTION
Due to most likely use of Bacillus anthracis in biological terrorism agents, the rapid and sensitive detection of its spores is crucial in both taking prophylactic measures and proper treatment. This study aimed to develop an amperometric electrochemical immunosensor for the detection of B. anthracis spores.
METHODS
A new amperometric biosensor was designed using a combination of magnetic beads and multiplex screen-printed electrodes. This method measures changes in current intensity resulting from oxidation and reduction in the working electrode directly to spore concentrations.
RESULTS
A standard curve was formed to test the number of live spores between 2 × 102-2 × 104 spores/ml concentrations. LOD and LOQ values were found to be 92 and 272 spores/ml, respectively. No cross-reactions were seen for Bacillus subtilis, Bacillus cereus and Bacillus thuringiencis spores.
CONCLUSIONS
It is shown that the designed Anthrax immunosensor has high sensitivity and selectivity with rapid detection results.
简介
由于炭疽杆菌极有可能被用作生物恐怖主义制剂，因此快速、灵敏地检测其孢子至关重要，这对于采取预防措施和适当治疗都非常重要。本研究旨在开发一种用于检测炭疽杆菌孢子的电流式电化学免疫传感器。
方法
采用磁珠和多重丝网印刷电极组合的方式设计了一种新的电流式生物传感器。该方法通过测量工作电极上的氧化还原反应引起的电流强度变化来直接检测孢子浓度。
结果
针对 2×102-2×104 个/ml 浓度范围内的活孢子数量，形成了标准曲线。检测限和定量限分别为 92 和 272 个/ml。该传感器对枯草芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌的孢子均无交叉反应。
结论
设计的炭疽杆菌免疫传感器具有高灵敏度和选择性，检测结果快速。
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