Ku Keun Bon, Chae Jihwan, Park Won Hyung, La Jeongwoo, Lee Seung S, Lee Heung Kyu
Laboratory of Host Defenses, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon, 34141, Republic of Korea.
Graduate School of Medical Science and Engineering, KAIST, Daejeon, 34141, Republic of Korea.
Lab Anim Res. 2024 Aug 13;40(1):28. doi: 10.1186/s42826-024-00214-6.
Water electrospray technology has been developed and extensively studied for its physical properties and potential application as a non-chemical biocide against airborne pathogens. However, there are still concerns regarding the safety and potential toxicity of inhaling water electrospray (WE) particles. To address these potential hazards and offer insights into the impact of WE on humans, we analyzed the immunopathological response to WE by employing an intranasal challenge C57BL/6 mouse model. This analysis aimed to compare the effects of WE with those of sodium hypochlorite (SH), a well-known biocidal agent.
The study findings suggest that the WE did not trigger any pathological immune reactions in the intranasal-challenged C57BL/6 mouse model. Mice challenged with WE did not experience body weight loss, and there was no increase in inflammatory cytokine production compared to SH-treated mice. Histopathological analysis revealed that WE did not cause any damage to the lung tissue. In contrast, mice treated with SH exhibited significant lung tissue damage, characterized by the infiltration of neutrophils and eosinophils. Transcriptomic analysis of lung tissue further confirmed the absence of a pathological immune response in mice treated with WE compared to those treated with SH. Upon intranasal challenge with WE, the C57BL/6 mouse model did not show any evidence of immunopathological damage.
The results of this study suggest that WE is a safe technology for disinfecting airborne pathogens. It demonstrated little to no effect on immune system activation and pathological outcomes in the intranasal challenge C57BL/6 mouse model. These findings not only support the potential use of WE as an effective and safe method for air disinfection but also highlight the value of the intranasal challenge of the C57BL/6 mouse model in providing significant immunopathological insights for assessing the inhalation of novel materials for potential use.
水喷雾技术因其物理特性以及作为针对空气传播病原体的非化学杀菌剂的潜在应用而得到开发和广泛研究。然而，对于吸入水喷雾（WE）颗粒的安全性和潜在毒性仍存在担忧。为了解决这些潜在危害并深入了解WE对人类的影响，我们通过采用鼻内激发C57BL/6小鼠模型分析了对WE的免疫病理反应。该分析旨在比较WE与著名杀菌剂次氯酸钠（SH）的效果。
研究结果表明，在鼻内激发的C57BL/6小鼠模型中，WE未引发任何病理性免疫反应。与SH处理的小鼠相比，接受WE激发的小鼠体重未减轻，炎症细胞因子产生也未增加。组织病理学分析显示，WE未对肺组织造成任何损伤。相比之下，用SH处理的小鼠表现出明显的肺组织损伤，其特征为中性粒细胞和嗜酸性粒细胞浸润。肺组织的转录组分析进一步证实，与SH处理的小鼠相比，接受WE处理的小鼠不存在病理性免疫反应。在用WE进行鼻内激发后，C57BL/6小鼠模型未显示任何免疫病理损伤的迹象。
本研究结果表明，WE是一种用于消毒空气传播病原体的安全技术。在鼻内激发C57BL/6小鼠模型中，它对免疫系统激活和病理结果几乎没有影响。这些发现不仅支持将WE作为一种有效且安全的空气消毒方法的潜在用途，还突出了C57BL/6小鼠模型鼻内激发在为评估新型潜在吸入材料提供重要免疫病理见解方面的价值。
