School of Chemical Science & Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai, 200092, China.
College of Biological and Environmental Engineering, Shandong University of Aeronautics, Binzhou, 256600, China.
Biochem Biophys Res Commun. 2024 Dec 10;737:150509. doi: 10.1016/j.bbrc.2024.150509. Epub 2024 Aug 8.
Salt stress is one of the significant environmental stresses that severely affect plant growth and development. Here, we report quantitative N-glycoproteomics characterization of differential N-glycosylation in Sorghum bicolor under low, median and high salinity stress. 21,621 intact N-glycopeptides coming from the combination of 127 N-glycan structures on 6574 N-glycosites from 5321 proteins were identified; differential N-glycosylation was observed for 682 N-glycoproteins which are mainly involved in the pathways of biosynthesis of secondary metabolites, biosynthesis of amino acids and several metabolic pathways. 41 N-glycan structures modifying on 338 N-glycopeptides from 122 glycoproteins were co-quantified and deregulated under at least one salt stress, including enzymes of energy production and carbohydrate metabolisms, cell wall organization related proteins, glycosyltransferases and so on. Intriguingly, with increasing salt concentration, there was an increase in the percentage of complex N-glycans on the altered N-glycopeptides. Furthermore, the observation of glycoproteins with distinct salt sensitivity is noteworthy, particularly the upregulated hyposensitive glycoproteins that predominantly undergo complex N-glycan modification. This is the first N-glycoproteome description of salt stress response at the intact N-glycopeptide level in sorghum and a further validation of data reported here would likely provide deeper insights into the stress physiology of this important crop plant.
盐胁迫是严重影响植物生长和发育的主要环境胁迫之一。在这里，我们报告了高粱在低盐、中盐和高盐胁迫下差异 N-糖基化的定量 N-糖基蛋白质组学特征。共鉴定到 21621 个完整的 N-糖肽，它们来自 6574 个 N-糖基化位点上的 127 个 N-聚糖结构，这些 N-糖基化位点来自 5321 个蛋白质；观察到 682 个 N-糖蛋白发生了差异糖基化，这些糖蛋白主要参与次生代谢物的生物合成途径、氨基酸的生物合成和几种代谢途径。在至少一种盐胁迫下，共定量和下调了 122 个糖蛋白的 338 个 N-糖肽上的 41 个 N-聚糖结构，包括能量产生和碳水化合物代谢的酶、细胞壁组织相关蛋白、糖基转移酶等。有趣的是，随着盐浓度的增加，改变的 N-糖肽上复杂 N-聚糖的百分比增加。此外，观察到具有不同盐敏感性的糖蛋白是值得注意的，特别是上调的低敏感糖蛋白，它们主要经历复杂 N-聚糖修饰。这是高粱在完整 N-糖肽水平上对盐胁迫反应的第一个 N-糖基蛋白质组描述，对这里报道的数据的进一步验证可能会深入了解这种重要作物的应激生理学。
