School of Life Sciences, Nanchang University, Nanchang, China.
School of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, China.
Food Funct. 2024 Aug 27;15(17):8916-8934. doi: 10.1039/d4fo03083d.
Rosacea, a chronic inflammatory dermatological condition, is characterized by facial erythema and pustules. Recent investigations have delved into the interplay between the gut microbiota and rosacea pathogenesis, unveiling promising avenues for therapeutic intervention. In this study, we screened and isolated strains 23-006 and 23-008 from the feces of healthy volunteers and evaluated the intervention effects of probiotics on rosacea by constructing an LL37 induced rosacea-like mouse model. Our results showed that both 23-006 and 23-008 were probiotic strains with favourable properties. In specific, we observed that both 23-006 and 23-008 alleviated skin lesions, reduced skin inflammatory infiltrates, and decreased the expression of inflammatory factors in mice, with the combination of 23-006 and 23-008 having the most significant effect. Moreover, the combination of strains reduced the expression of cathelicidin LL37 and rosacea-associated factors by inhibiting the TLR2/MyD88/NF-κB pathway. The 16S rRNA analysis showed that the combination enhanced the intestinal barrier, restored intestinal microbiota homeostasis, and up-regulated the abundance of while down-regulating the abundance of and . We also explored the effects of postbiotics of 23-006 and 23-008 on rosacea. While postbiotics could also ameliorate the rosacea-like phenotype in mice the TLR2/MyD88/NF-κB pathway, the effects were not as pronounced as those observed with probiotic treatment. However, the postbiotics still enhanced the intestinal barrier, up-regulated the abundance, and modulated the intestinal microbiota. In conclusion, our study revealed that 23-006 and 23-008 improved rosacea by regulating the TLR2/MyD88/NF-κB pathway and intestinal microbiota, providing a theoretical basis for the treatment of rosacea.
酒渣鼻是一种慢性炎症性皮肤病，其特征为面部红斑和脓疱。最近的研究深入探讨了肠道微生物群与酒渣鼻发病机制之间的相互作用，揭示了治疗干预的有前途的途径。在这项研究中，我们从健康志愿者的粪便中筛选和分离了菌株 23-006 和 23-008，并通过构建 LL37 诱导的酒渣鼻样小鼠模型来评估益生菌对酒渣鼻的干预效果。我们的结果表明，23-006 和 23-008 都是具有良好特性的益生菌株。具体来说，我们观察到 23-006 和 23-008 均能减轻皮肤损伤，减少皮肤炎症浸润，并降低小鼠炎症因子的表达，而 23-006 和 23-008 的联合作用效果最为显著。此外，该联合菌株通过抑制 TLR2/MyD88/NF-κB 通路降低了抗菌肽 LL37 和酒渣鼻相关因子的表达。16S rRNA 分析表明，该联合菌株增强了肠道屏障，恢复了肠道微生物群的平衡，并上调了 的丰度，同时下调了 和 的丰度。我们还探索了 23-006 和 23-008 的后生元对酒渣鼻的影响。后生元虽然也能改善小鼠的酒渣鼻样表型，但对 TLR2/MyD88/NF-κB 通路的抑制作用不如益生菌治疗明显。然而，后生元仍能增强肠道屏障，上调 的丰度，并调节肠道微生物群。总之，我们的研究表明，23-006 和 23-008 通过调节 TLR2/MyD88/NF-κB 通路和肠道微生物群改善了酒渣鼻，为酒渣鼻的治疗提供了理论依据。
