Key Laboratory of Integrated Pest Management on Crops in East China, Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P. R. China.
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, P. R. China.
J Agric Food Chem. 2024 Aug 28;72(34):18816-18823. doi: 10.1021/acs.jafc.4c02866. Epub 2024 Aug 15.
The fall armyworm (FAW) is a serious agricultural pest and has developed resistance to multiple insecticides. It is necessary to introduce novel insecticide(s) for controlling FAW. Isocycloseram is a completely novel isoxazoline insecticide. However, its activity and mode of action against FAW have not been reported. In this study, isocycloseram exhibited a higher insecticidal activity (LC = 0.26 mg/kg) than fipronil (LC = 7.72 mg/kg) against FAW. The median inhibitory concentration (IC) of isocycloseram (IC = 8.52 nM) was almost equal to that of the desmethyl-broflanilide (IC = 7.32 nM) to the RDL1 receptor. The IC of isocycloseram to the RDL2 receptor was 11.13 nM, which was obviously less than that of desmethyl-broflanilide, dieldrin, fipronil, fluxametamide. Compared with the RDL2 receptor, the RDL1 receptor exhibited higher sensitivity to GABAergic insecticides. The recombinant GluCl receptor was successfully stimulated by l-glutamate; however, the currents were low and weakly inhibited by isocycloseram at 10 μM. In conclusion, our results provided the theoretical basis for usage of GABAergic insecticides for controlling FAW.
秋粘虫是一种严重的农业害虫，已经对多种杀虫剂产生了抗性。因此，有必要引入新型杀虫剂来防治秋粘虫。异环虫酰胺是一种全新的异噁唑啉类杀虫剂。然而，其对秋粘虫的活性和作用模式尚未有报道。在本研究中，异环虫酰胺对秋粘虫的杀虫活性（LC = 0.26 mg/kg）高于氟虫腈（LC = 7.72 mg/kg）。异环虫酰胺对 RDL1 受体的半数抑制浓度（IC = 8.52 nM）几乎与去甲溴虫腈（IC = 7.32 nM）相当。异环虫酰胺对 RDL2 受体的 IC 为 11.13 nM，明显低于去甲溴虫腈、狄氏剂、氟虫腈、唑虫酰胺。与 RDL2 受体相比，RDL1 受体对 GABA 能杀虫剂表现出更高的敏感性。重组 GluCl 受体可被 l-谷氨酸成功激活；然而，当浓度为 10 μM 时，异环虫酰胺对电流的激活作用较弱。综上所述，本研究结果为 GABA 能杀虫剂防治秋粘虫提供了理论依据。
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