Institute for Plant Protection, National Agriculture and Food Research Organization, Tsukuba, Japan.
Virus Genes. 2024 Dec;60(6):674-683. doi: 10.1007/s11262-024-02098-z. Epub 2024 Aug 16.
The complete genome sequence of Orthotospovirus tomatozonae (tomato zonate spot virus, TZSV) isolated in Japan was determined and compared with that of Chinese isolates. The lengths of the S, M, and L segments of the RNA genomes of the Japanese isolate (TZSV-TZ1-3) were 3194, 4675, and 8916 nucleotides, respectively, which were similar to the Chinese isolates. Moreover, the eight motifs on the RNA-dependent RNA polymerase (RdRp) gene were conserved in both TZSV-TZ1-3 and Chinese TZSV isolates (TZSV-Bidens and TZSV-Tomato-YN). The nucleotide identity of the genes among the TZSV isolates was more than 94%, indicating low diversity among viruses. The phylogenetic analysis and the prediction of the cleavage sites in the glycoprotein showed that the TZSV-TZ1-3 isolate was closely related to TZSV-Tomato-YN isolated from China. However, there were unique frameshifts and deletions on the RdRp and glycoprotein genes of the TZSV-Tomato-YN isolate, suggesting that both isolates were genetically distinct. The findings of this study indicate that the TZSV-TZ1-3 isolate originated in China and show the sequence diversity among TZSV isolates.
日本分离的番茄斑萎病毒（番茄斑萎病毒，TZSV）的全基因组序列已被确定，并与中国分离株进行了比较。日本分离株（TZSV-TZ1-3）的 RNA 基因组 S、M 和 L 片段的长度分别为 3194、4675 和 8916 个核苷酸，与中国分离株相似。此外，RNA 依赖的 RNA 聚合酶（RdRp）基因上的 8 个基序在 TZSV-TZ1-3 和中国 TZSV 分离株（TZSV-Bidens 和 TZSV-Tomato-YN）中均保守。TZSV 分离株之间基因的核苷酸同一性超过 94%，表明病毒之间的多样性较低。系统进化分析和糖蛋白裂解位点的预测表明，TZSV-TZ1-3 分离株与中国分离的 TZSV-Tomato-YN 分离株密切相关。然而，TZSV-Tomato-YN 分离株的 RdRp 和糖蛋白基因存在独特的移码和缺失，表明这两个分离株在遗传上是不同的。本研究的结果表明，TZSV-TZ1-3 分离株起源于中国，并显示了 TZSV 分离株之间的序列多样性。
