唾液中的脆弱拟杆菌：探究其与溃疡性结肠炎的关联

Bacteroides fragilis in saliva: investigating links with ulcerative colitis.

作者信息

Zamani Samin, Besharat Sima, Behnampour Nasser, Behnam Armina, Asgari Negar, Mortazavi Nazanin

机构信息

Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

出版信息

Braz J Microbiol. 2024 Dec;55(4):3691-3698. doi: 10.1007/s42770-024-01484-x. Epub 2024 Aug 19.

DOI:10.1007/s42770-024-01484-x

PMID:39155343

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11711552/

Abstract

BACKGROUND

Ulcerative colitis (UC) is a long-term bowel inflammation of unknown cause. Recent research points to gut microbiota, especially Enterotoxigenic Bacteroides fragilis (ETBF), in UC's development. This study examined the presence of Bacteroides fragilis (B. fragilis) and ETBF in the saliva of UC patients and Healthy Controls (HCs) in Iran.

METHODS

A total of 40 UC patients and 40 healthy controls were included in the study. Saliva samples were collected and analyzed for the presence of B. fragilis and ETBF using real-time polymerase chain reaction (PCR).

RESULTS

B. fragilis was more prevalent in HCs (70%) than UC patients (67.5%), but not significantly (p = 0.809). ETBF was significantly more prevalent in UC patients (50%) than HCs (10%) (p < 0.0001). The mean count of B. fragilis was higher in UC patients, but not significantly (p = 0.47). However, the mean count of ETBF was significantly higher in UC patients (p = 0.000089). In terms of gender, the number of B. fragilis in women was not significant (p = 0.16), but the number of ETBF was significantly higher in women with UC (p = 0.000458). For men, no significant differences were observed.

CONCLUSIONS

The present study suggest a higher prevalence of B. fragilis observed in UC patients compared to HCs. Further research is needed to confirm these findings and explore potential mechanisms underlying this association.

摘要

背景

溃疡性结肠炎（UC）是一种病因不明的慢性肠道炎症。近期研究表明肠道微生物群，尤其是产肠毒素脆弱拟杆菌（ETBF），在UC的发病过程中起作用。本研究检测了伊朗UC患者和健康对照者（HCs）唾液中脆弱拟杆菌（B. fragilis）和ETBF的存在情况。

方法

本研究共纳入40例UC患者和40例健康对照者。收集唾液样本，采用实时聚合酶链反应（PCR）分析B. fragilis和ETBF的存在情况。

结果

脆弱拟杆菌在HCs中的患病率（70%）高于UC患者（67.5%），但差异无统计学意义（p = 0.809）。ETBF在UC患者中的患病率（50%）显著高于HCs（10%）（p < 0.0001）。UC患者中脆弱拟杆菌的平均计数较高，但差异无统计学意义（p = 0.47）。然而，UC患者中ETBF的平均计数显著更高（p = 0.000089）。在性别方面，女性中脆弱拟杆菌的数量差异无统计学意义（p = 0.16），但UC女性患者中ETBF的数量显著更高（p = 0.000458）。男性中未观察到显著差异。