Kazi Kira J, English Cole D, Ivantsova Emma, Souders Christopher L, Martyniuk Christopher J
Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA.
UF Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, USA.
Data Brief. 2024 Jul 16;55:110756. doi: 10.1016/j.dib.2024.110756. eCollection 2024 Aug.
Citalopram, a selective serotonin reuptake inhibitor (SSRI), is often detected in aquatic ecosystems. In this investigation, developing zebrafish were continuously exposed to one nominal concentration of either 0, 10, or 1000 µg/L citalopram for 7 days. Ribonucleic acids were then extracted from zebrafish for RNA-sequencing using the NovoSeq 6000 (Illumina). Clean reads were obtained following the removal of both the adapter and poly-N sequences. Alignment and differential gene expression analysis was conducted using programs HISAT2 and StringTie assembler. Data were converted to FPKM to quantify differentially expressed transcripts. Significant clinical subnetworks enriched following citalopram exposure included sympathetic nerve activity, blood pressure, vascular tone, and arterial pressure. Regulated transcripts were related to diseases such as mechanical hyperalgesia, pain, inflammatory pain, obstructive hypertrophic cardiomyopathy, fatigue, Diamond-Blackfan anemia, and hypertrophic cardiomyopathy. Following exposure to 10 µg/L citalopram, several transcripts were linked to brain dysfunction like prostaglandin-endoperoxide synthase 2, microtubule associated protein tau, cathepsin B, and dystrophin. Genes related to cardiac dysfunction were altered in zebrafish following exposure to 1000 µg/L citalopram. Using literature and databases that describe gene interactions, molecular networks (clinical and disease networks) were constructed to understand effects of citalopram.
西酞普兰是一种选择性5-羟色胺再摄取抑制剂（SSRI），经常在水生生态系统中被检测到。在本研究中，发育中的斑马鱼连续7天暴露于0、10或1000μg/L的西酞普兰标称浓度之一。然后从斑马鱼中提取核糖核酸，使用NovoSeq 6000（Illumina）进行RNA测序。去除接头和多聚N序列后获得了干净的 reads。使用HISAT2和StringTie汇编程序进行比对和差异基因表达分析。数据被转换为FPKM以量化差异表达的转录本。西酞普兰暴露后富集的重要临床子网包括交感神经活动、血压、血管张力和动脉压。受调控的转录本与机械性痛觉过敏、疼痛、炎性疼痛、梗阻性肥厚型心肌病、疲劳、先天性纯红细胞再生障碍性贫血和肥厚型心肌病等疾病有关。暴露于10μg/L西酞普兰后，一些转录本与脑功能障碍有关，如前列腺素内过氧化物合酶2、微管相关蛋白tau、组织蛋白酶B和肌营养不良蛋白。暴露于1000μg/L西酞普兰后，斑马鱼中与心脏功能障碍相关的基因发生了改变。利用描述基因相互作用的文献和数据库，构建了分子网络（临床和疾病网络）以了解西酞普兰的作用。
